PTEN/P16 INHIBITS INFLAMMATORY RESPONSE AND REDUCES INTESTINAL METAPLASIA IN GASTRIC MUCOSA OF CHRONIC ATROPHIC GASTRITIS AND PREVENTS THE CARCINOGENESIS

被引:1
作者
Chen, Kailiang [1 ]
Liu, Yu [2 ]
Wang, Chaoqun [3 ]
Xu, Jiafang [4 ]
Gong, Jian [5 ]
机构
[1] Hainan Med Univ, Dept Ultrasound, Affiliated Hosp 1, Haikou 570102, Hainan, Peoples R China
[2] Hainan Med Univ, Dept Breast & Thorac Ontol Surg, Affiliated Hosp 1, Haikou 570102, Hainan, Peoples R China
[3] Hainan Med Univ, Dept Nucl Med, Hainan Gen Hosp, Hainan Affiliated Hosp, 31 Longhua Rd, Haikou 570311, Hainan, Peoples R China
[4] Hainan Med Univ, Reprod Med Ctr, Affiliated Hosp 1, 31 Longhua Rd, Haikou 570102, Hainan, Peoples R China
[5] Taian City Cent Hosp, Dept Gastroenterol, Tai An 271000, Shandong, Peoples R China
来源
ACTA MEDICA MEDITERRANEA | 2021年 / 37卷 / 06期
关键词
chronic atrophic gastritis; gastric cancer; PTEN; p16; inflammatory reaction; intestinal metaplasia; CANCER; EXPRESSION; CELLS;
D O I
10.19193/0393-6384_2021_6_499
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Objective: To investigate the protective roles of phosphate and tension homology deleted on chromsome ten (PTEN)/p16 in gastric cancer (GC) progression by reducing the production of proinflammatory cytokines and intestinal metaplasia (IM) in gastric mucosa of chronic atrophic gastritis (CAG). Methods: Thirty normal persons (normal group), 30 CAG patients with small intestinal metaplasia (SIM) (CAG+ SIM group), 30 CAG patients with colonic intestinal metaplasia (CIM) (CAG+ CIM group), and 30 GC patients (GC group) were enrolled. The gastric mucosa tissues were collected. The expressions of PTEN, p16, interleukin-1 beta (IL-1 beta), tumor necrosis factor-alpha (TNF-alpha), caudal type homeobox transcription factor (CDX) 1 and CDX2 were detected. The correlations of these indexes in GC tissues were analyzed. Moreover, the expressions of PTEN and p16 in GC cell lines were detected. After over-expressed PTEN and p16 in GC cells were constructed successfully, the expression changes of IL-1 beta, TNF-alpha, CDX1 and CDX2 were measured, and the roles of PTEN and p16 in GC cell proliferation and tumor growth abilities were analyzed. Results: The expression levels of PTEN and p16 were decreased while IL-1 beta and TNF-alpha, CDX1 and CDX2 were increased in gastric mucosa in CAG+ SIM, CAG+ CIM and GC groups, compared with normal group. The degrees of these decreased and increased tendencies of above genes were associated with the degree of IM and canceration of gastric mucosa. The levels of IL-1 beta, TNF-alpha, CDX1 and CDX2 were negatively correlated with PTEN and p16 in GC specimens. Besides, PTEN and p16 expressions were declined in diverse GC cell lines, and the over-expression of PTEN and p16 could restrain the expressions of IL-1 beta, TNF-alpha, CDX1 and CDX2 as well as inhibit the GC cell proliferation and tumor growth. Conclusion: PTEN and p16 can inhibit the inflammatory response and reduce the IM in gastric mucosa of CAG and prevent the occurrence of carcinogenesis.
引用
收藏
页码:3171 / 3177
页数:7
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