Assessment of antiviral activity and mechanism of rhein on newcastle disease virus (La Sota strain IV) in vitro

被引：7

作者：

Hu, Yanchun ^{[1
]}

Okyere, Samuel Kumi ^{[1
]}

Xu, Ruiguang ^{[1
]}

Peng, Guangneng ^{[1
]}

Ren, Zhihua ^{[1
]}

Deng, Junliang ^{[1
]}

Jia, Yan ^{[2
]}

机构：

[1] Sichuan Agr Univ, Coll Vet Med, Key Lab Anim Dis & Human Hlth Sichuan Prov, Wenjiang, Sichuan, Peoples R China

[2] Jiaxing Vocat Tech Coll, Agr & Environm Branch, Jiaxing, Zhejiang, Peoples R China

来源：

NATURAL PRODUCT RESEARCH | 2022年 / 36卷 / 05期

关键词：

Chicken embryo fibroblasts; newcastle disease; traditional Chinese medicine; rhein;

D O I：

10.1080/14786419.2021.1878515

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Current research is focused on the development of drug candidates from natural products. Rhein a Traditional Chinese Medicine (TCM) from Polygonaceae (rhubarb) has exhibited antioxidant, anti-inflammatory and anticancer activities, however no work has reported its antiviral potential, thus this study was performed to investigate the antiviral activities of rhein on new castle disease virus (NDV) in vitro. NDV infection of chicken embryo fibroblasts (CEFs) was prepared using 10-day-old specific pathogen free chicken embryos. Cytotoxicity and anti-viral activities of rhein were assessed using the MTT method. The interaction between NDV and cell membrane proteins were also detected using virus overlay protein binding assay (VOPBA). In addition NDV genes expressions in CEFs were measured using real-time fluorescent quantitative (RTFQ) PCR. The results showed that rhein effectively inhibit NDV activities maximal safe concentration of 0.125 mg/ml. This finding indicated that, rhein could be used as future antiviral drug against NDV.

引用

页码：1400 / 1404

页数：5

共 14 条

[11] Rhein, a Natural Anthraquinone Derivative, Attenuates the Activation of Pancreatic Stellate Cells and Ameliorates Pancreatic Fibrosis in Mice with Experimental Chronic Pancreatitis [J].

Tsang, Siu Wai ;

Zhang, Hongjie ;

Lin, Chengyuan ;

Xiao, Haitao ;

Wong, Michael ;

Shang, Hongcai ;

Yang, Zhi-Jun ;

Lu, Aiping ;

Yung, Ken Kin-Lam ;

Bian, Zhaoxiang .

PLOS ONE, 2013, 8 (12)

[12] Bioactive natural compounds against human coronaviruses: a review and perspective [J].

Xian, Yanfang ;

Zhang, Juan ;

Bian, Zhaoxiang ;

Zhou, Hua ;

Zhang, Zhenbiao ;

Lin, Zhixiu ;

Xu, Hongxi .

ACTA PHARMACEUTICA SINICA B, 2020, 10 (07) :1163-1174

[13] Anti-NDV activity of 9-oxo10,11-dehydroageraphorone extracted from Eupatorium adenophorum Spreng in vitro [J].

Xu, Ruiguang ;

Weng, Jiahua ;

Hu, Liwen ;

Peng, Guangneng ;

Ren, Zhihua ;

Deng, Junliang ;

Jia, Yan ;

Wang, Chengmin ;

He, Hongxuan ;

Hu, Yanchun .

NATURAL PRODUCT RESEARCH, 2018, 32 (18) :2244-2247

[14] Flavone ingredients can synergistically inhibit NDV infecting cell and improve ND vaccine's protective rate [J].

Zhang Yuqing ;

Sun Zhan'ao ;

Liu Jiaguo ;

Wang Deyun ;

Zhang Baokang ;

Yi Fang ;

Fan Yunpeng ;

Liu Dan ;

Liu Xu ;

Liu Cui ;

Hu Yuanliang .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2012, 51 (03) :201-208

← 1 2 →