Cloning, sequencing, and expression in Escherichia coli of the new gene encoding β-1,3-xylanase from a marine bacterium, Vibrio sp strain XY-214

被引：42

作者：

Araki, T ^{[1
]}

Hashikawa, S ^{[1
]}

Morishita, T ^{[1
]}

机构：

[1] Mie Univ, Fac Bioresources, Tsu, Mie 5148507, Japan

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2000年 / 66卷 / 04期

关键词：

D O I：

10.1128/AEM.66.4.1741-1743.2000

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The Vibrio sp. strain XY-214 beta-1,3-xylanase gene cloned in Escherichia coli DH5 alpha consisted of an open reading frame of 1,383 nucleotides encoding a protein of 460 amino acids with a molecular mass of 51,323 Da and had a signal peptide of 22 amino acids. The transformant enzyme hydrolyzed beta-1,3-xylan to produce several xylooligosaccharides.

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页码：1741 / 1743

页数：3

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