Separation of enantiomers of chiral weak acids with polysaccharide-based chiral columns and aqueous-organic mobile phases in high-performance liquid chromatography: Typical reversed-phase behavior?

被引：61

作者：

Matarashvili, Iza ^{[1
]}

Ghughunishvili, Darejan ^{[2
]}

Chankvetadze, Lali ^{[1
]}

Takaishvili, Nino ^{[1
]}

Khatiashvili, Tamar ^{[1
]}

Tsintsadze, Maia ^{[2
]}

Farkas, Tivadar ^{[3
]}

Chankvetadze, Bezhan ^{[1
]}

机构：

[1] Tbilisi State Univ, Sch Exact & Nat Sci, Inst Phys & Analyt Chem, Chavchavadze Ave 3, GE-0179 Tbilisi, Georgia

[2] Georgian Tech Univ, Fac Chem, Kostava Str 77, GE-0102 Tbilisi, Georgia

[3] Phenomenex Inc, 411 Madrid Ave, Torrance, CA 90501 USA

来源：

JOURNAL OF CHROMATOGRAPHY A | 2017年 / 1483卷

基金：

美国国家科学基金会;

关键词：

Enantioseparations; Chiral HPLC; Polysaccharide-based chiral stationary phases; Reversal of enantiomer elution order; STATIONARY PHASES; HPLC SEPARATION; RETENTION; ENANTIOSEPARATION; EMPHASIS; ORDER;

D O I：

10.1016/j.chroma.2016.12.064

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

When polysaccharide-based chiral columns are used in combination with aqueous-organic mobile phases for the separation of enantiomers in high-performance liquid chromatography the separation mode is commonly called reversed-phase in analogy to achiral separations. In several earlier and recent studies on neutral and basic chiral analytes it was shown by our and other groups that due to multiple type of interactions involved in selector-selectand binding and enantioselective recognition with polysaccharide derivatives, the above mentioned separation system may not always behave like a reversed-phase system. In the present study additional examples of non-reversed-phase behavior are described for the first time for weak acidic chiral analytes. In addition, the reversal of enantiomer elution order was observed again for the first time for several analytes based on water-content in the mobile phase. (C) 2016 Elsevier B.V. All rights reserved.

引用

页码：86 / 92

页数：7

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