Differential expression and prognostic value of long non-coding RNA in HPV-negative head and neck squamous cell carcinoma

被引：34

作者：

Sulsal-Ul Haque ^{[1
]}

Niu, Liang ^{[2
]}

Kuhnell, Damaris ^{[2
]}

Hendershot, Jacob ^{[2
]}

Biesiada, Jacek ^{[2
]}

Niu, Wen ^{[2
]}

Hagan, Matthew C. ^{[3
]}

Kelsey, Karl T. ^{[4
,5
]}

Casper, Keith A. ^{[6
]}

Wise-Draper, Trisha M. ^{[1
,7
]}

Medvedovic, Mario ^{[2
]}

Langevin, Scott M. ^{[2
]}

机构：

[1] Univ Cincinnati, Coll Med, Dept Internal Med, Div Hematol Oncol, Cincinnati, OH USA

[2] Univ Cincinnati, Coll Med, Dept Environm Hlth, Cincinnati, OH 45267 USA

[3] Univ Cincinnati, Coll Med, Dept Pathol & Lab Med, Cincinnati, OH USA

[4] Brown Univ, Dept Epidemiol, Providence, RI 02912 USA

[5] Brown Univ, Dept Pathol & Lab Med, Providence, RI 02912 USA

[6] Univ Michigan, Dept Otolaryngol, Ann Arbor, MI 48109 USA

[7] Univ Cincinnati, Coll Med, Dept Canc Biol, Cincinnati, OH USA

来源：

HEAD AND NECK-JOURNAL FOR THE SCIENCES AND SPECIALTIES OF THE HEAD AND NECK | 2018年 / 40卷 / 07期

关键词：

head and neck cancer; head and neck squamous cell carcinoma (HNSCC); long noncoding RNA (lncRNA); noncoding RNA (ncRNA); survival; The Cancer Genome Atlas (TCGA); LUNG-CANCER; COMPETING RISKS; POOR-PROGNOSIS; SURVIVAL; HOTAIR; MEG3; IDENTIFICATION; PROLIFERATION; PROGRESSION; METASTASIS;

D O I：

10.1002/hed.25136

中图分类号：

R76 [耳鼻咽喉科学];

学科分类号：

100213 ;

摘要：

Background: Long non-coding RNA (lncRNA) has emerged as a new avenue of interest due to its various biological functions in cancer. Abnormal expression of lncRNA has been reported in other malignancies but has been understudied in head and neck squamous cell carcinoma (HNSCC). Methods: The lncRNA expression was interrogated via quantitative real-time polymerase chain reaction (qRT-PCR) array for 19 human papillomavirus (HPV)negative HNSCC tumor-normal pairs. The Cancer Genome Atlas (TCGA) was used to validate these results. The association between differentially expressed lncRNA and survival outcomes was analyzed. Results: Differential expression was validated for 5 lncRNA (SPRY4-IT1, HEIH, LUCAT1, LINC00152, and HAND2-AS1). There was also an inverse association between MEG3 expression (not significantly differentially expressed in TCGA tumors but highly variable expression) and 3-year recurrence-free survival (RFS). Conclusion: We identified and validated differential expression of 5 lncRNA in HPV-negative HNSCC. Low MEG3 expression was associated with favorable 3-year RFS, although the significance of this finding remains unclear.

引用

页码：1555 / 1564

页数：10

共 39 条

[1]

[Anonymous], BMC CANC

[2] From RNA isolation to microarray analysis: Comparison of methods in FFPE tissues [J].

Belder, Nevin ;

Coskun, Oznur ;

Erdogan, Beyza Doganay ;

Ilk, Ozlem ;

Savas, Berna ;

Ensari, Arzu ;

Ozdag, Hilal .

PATHOLOGY RESEARCH AND PRACTICE, 2016, 212 (08) :678-685

[3] CONTROLLING THE FALSE DISCOVERY RATE - A PRACTICAL AND POWERFUL APPROACH TO MULTIPLE TESTING [J].

BENJAMINI, Y ;

HOCHBERG, Y .

JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES B-STATISTICAL METHODOLOGY, 1995, 57 (01) :289-300

[4] Patterns of failure, prognostic factors and survival in locoregionally advanced head and neck cancer treated with concomitant chemoradiotherapy: a 9-year, 337-patient, multi-institutional experience [J].

Brockstein, B ;

Haraf, DJ ;

Rademaker, AW ;

Kies, MS ;

Stenson, KM ;

Rosen, F ;

Mittal, BB ;

Pelzer, H ;

Fung, BB ;

Witt, ME ;

Wenig, B ;

Portugal, L ;

Weichselbaum, RW ;

Vokes, EE .

ANNALS OF ONCOLOGY, 2004, 15 (08) :1179-1186

[5] The Noncoding RNA Revolution-Trashing Old Rules to Forge New Ones [J].

Cech, Thomas R. ;

Steitz, Joan A. .

CELL, 2014, 157 (01) :77-94

[6] Long intergenic non-coding RNA 00152 promotes lung adenocarcinoma proliferation via interacting with EZH2 and repressing IL24 expression [J].

Chen, Qin-nan ;

Chen, Xin ;

Chen, Zhen-yao ;

Nie, Feng-qi ;

Wei, Chen-chen ;

Ma, Hong-wei ;

Wan, Li ;

Yan, Shuai ;

Ren, Sheng-nan ;

Wang, Zhao-xia .

MOLECULAR CANCER, 2017, 16

[7] TCGAbiolinks: an R/Bioconductor package for integrative analysis of TCGA data [J].

Colaprico, Antonio ;

Silva, Tiago C. ;

Olsen, Catharina ;

Garofano, Luciano ;

Cava, Claudia ;

Garolini, Davide ;

Sabedot, Thais S. ;

Malta, Tathiane M. ;

Pagnotta, Stefano M. ;

Castiglioni, Isabella ;

Ceccarelli, Michele ;

Bontempi, Gianluca ;

Noushmehr, Houtan .

NUCLEIC ACIDS RESEARCH, 2016, 44 (08) :e71

[8]

Cui XR, 2017, ONCOTARGET, V8, P19049, DOI 10.18632/oncotarget.14987

[9] Landscape of transcription in human cells [J].

Djebali, Sarah ;

Davis, Carrie A. ;

Merkel, Angelika ;

Dobin, Alex ;

Lassmann, Timo ;

Mortazavi, Ali ;

Tanzer, Andrea ;

Lagarde, Julien ;

Lin, Wei ;

Schlesinger, Felix ;

Xue, Chenghai ;

Marinov, Georgi K. ;

Khatun, Jainab ;

Williams, Brian A. ;

Zaleski, Chris ;

Rozowsky, Joel ;

Roeder, Maik ;

Kokocinski, Felix ;

Abdelhamid, Rehab F. ;

Alioto, Tyler ;

Antoshechkin, Igor ;

Baer, Michael T. ;

Bar, Nadav S. ;

Batut, Philippe ;

Bell, Kimberly ;

Bell, Ian ;

Chakrabortty, Sudipto ;

Chen, Xian ;

Chrast, Jacqueline ;

Curado, Joao ;

Derrien, Thomas ;

Drenkow, Jorg ;

Dumais, Erica ;

Dumais, Jacqueline ;

Duttagupta, Radha ;

Falconnet, Emilie ;

Fastuca, Meagan ;

Fejes-Toth, Kata ;

Ferreira, Pedro ;

Foissac, Sylvain ;

Fullwood, Melissa J. ;

Gao, Hui ;

Gonzalez, David ;

Gordon, Assaf ;

Gunawardena, Harsha ;

Howald, Cedric ;

Jha, Sonali ;

Johnson, Rory ;

Kapranov, Philipp ;

King, Brandon .

NATURE, 2012, 489 (7414) :101-108

[10] Toward a Shared Vision for Cancer Genomic Data [J].

Grossman, Robert L. ;

Heath, Allison P. ;

Ferretti, Vincent ;

Varmus, Harold E. ;

Lowy, Douglas R. ;

Kibbe, Warren A. ;

Staudt, Louis M. .

NEW ENGLAND JOURNAL OF MEDICINE, 2016, 375 (12) :1109-1112

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