Isolation and expression analysis of AGAMOUS-like genes from Eucalyptus grandis

被引：3

作者：

Kato, Tomohiko ^{[1
]}

Hibino, Takashi ^{[1
]}

机构：

[1] Oji Paper Co Ltd, Forestry Res Inst, Kameyama 5190212, Japan

来源：

PLANT BIOTECHNOLOGY | 2009年 / 26卷 / 01期

关键词：

AGAMOUS-like; Eucalyptus; MADS-box; MADS-BOX GENES; ARABIDOPSIS; EVOLUTION; FLOWERS; FAMILY;

D O I：

10.5511/plantbiotechnology.26.121

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this study, as a first step to achieve a goal to produce genetically-engineered sterile Eucalyptus, we focussed on the AGAMOUS-like genes which are especially involved in stamen formation. Three new distinct cDNA fragments (EgAGL1, EgAGL2 and EgAGL3) and two distinct genomic DNA fragments (EgAGL1 and EgAGL2) encoding MADS-box proteins were isolated from Eucalyptus grandis. In the present study, three genes have 62-74% homology with the Arabidopsis AGAMOUS (AG) gene in deduced amino acid level. EgAGL1, EgAGL2 and EgAGL3 were classified close to the AGAMOUS gene by phylogenic analysis, EgAGL1 and EgAGL2 were strongly expressed in flower buds, and consequently may regulate stamen formation in Eucalyptus.

引用

页码：121 / 124

页数：4

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