In order to obtain cell protein from the monosodium glutamate (MSG) waste water, the culture conditions for yeast were studied in this paper. The optimum condition was that: the liquid capacity was 60 mL/250mL flask, liquor dilution ratio of MSG waste water was 1:1, the culture time for liquid seed was 16 hours, inoculation rate was 8%, speed was 180 rpm, Temperature was 28 C. Cultured at the optimum conditions for 36h, 28.28g/L cell protein was obtained, 92.2% utilization of reducing sugar was reached, 71.1% removal rate of chemical oxygen demand was realized and 64.7 % removal rate of Kjeldahl nitrogen was achieved. The cell protein could be recovered, the results do spade work for after-treatment. Certain economic and environmental benefits could be obtained.