Immobilized soybean hull peroxidase for the oxidation of phenolic compounds in coffee processing wastewater

被引：66

作者：

Batista Chagas, Pricila Maria ^{[1
]}

Torres, Juliana Arriel ^{[1
]}

Silva, Maria Cristina ^{[2
]}

Correa, Angelita Duarte ^{[1
]}

机构：

[1] Univ Fed Lavras, Dept Quim, Lab Bioquim, BR-37200000 Lavras, MG, Brazil

[2] Ctr Fed Educ Tecnol Minas Gerais, BR-30421169 Belo Horizonte, MG, Brazil

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2015年 / 81卷

关键词：

Enzyme; Immobilization; Chitosan; Glutaraldehyde; Caffeic acid; Coffee processing wastewater; HORSERADISH-PEROXIDASE; POTENTIAL APPLICATIONS; ENZYMATIC REMOVAL; CHITOSAN; DECOLORIZATION; DEGRADATION; CHITIN; POLYMERIZATION; STABILIZATION; MICROSPHERES;

D O I：

10.1016/j.ijbiomac.2015.08.061

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Chitosan beads were prepared, using glutaraldehyde as a crosslinking agent for the immobilization of soybean hull peroxidase (SBP). The activity of free and immobilized SBP was studied. The optimum pH was 6.0 for both the free and immobilized enzyme; however, enzyme activity became more dependent on the temperature after immobilization. This study evaluated the potential use of immobilized and free enzyme in the oxidation of caffeic acid, of synthetic phenolic solution (SPS) and of total phenolic compounds in coffee processing wastewater (CPW). Some factors, such as reaction time, amount of H2O2 and caffeic acid were evaluated, in order to determine the optimum conditions for enzyme performance. Both enzymes showed a potential in the removal of caffeic acid, SPS and CPW, and immobilized SBP had the highest oxidation performance. The immobilized enzyme showed a potential of 50% in the oxidation of caffeic acid after 4 consecutive cycles. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：568 / 575

页数：8

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