Ectopic hbox12 Expression Evoked by Histone Deacetylase Inhibition Disrupts Axial Specification of the Sea Urchin Embryo

被引：17
作者：
Cavalieri, Vincenzo [1 ,2 ]
Spinelli, Giovanni [1 ]
机构：
[1] Univ Palermo, Dept Biol Chem & Pharmaceut Sci & Technol STEBICE, I-90133 Palermo, Italy
[2] Univ Palermo, Mediterranean Ctr Human Hlth Adv Biotechnol CHAB, I-90133 Palermo, Italy
来源：
PLOS ONE | 2015年 / 10卷 / 11期
关键词：
GENE REGULATORY NETWORKS; HOMEOBOX-CONTAINING GENE; ECTODERM CELLS AFFECTS; ORAL-ABORAL AXIS; TRICHOSTATIN-A; TRANSCRIPTIONAL NETWORKS; VALPROIC ACID; ACETYLATION; POTENT; METHYLATION;
D O I：
10.1371/journal.pone.0143860
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Dorsal/ventral patterning of the sea urchin embryo depends upon the establishment of a Nodal-expressing ventral organizer. Recently, we showed that spatial positioning of this organizer relies on the dorsal-specific transcription of the Hbox12 repressor. Building on these findings, we determined the influence of the epigenetic milieu on the expression of hbox12 and nodal genes. We find that Trichostatin-A, a potent and selective histone-deacetylases inhibitor, induces histone hyperacetylation in hbox12 chromatin, evoking broad ectopic expression of the gene. Transcription of nodal concomitantly drops, prejudicing dorsal/ventral polarity of the resulting larvae. Remarkably, impairing hbox12 function, either in a spatially-restricted sector or in the whole embryo, specifically rescues nodal transcription in Trichostatin-A-treated larvae. Beyond strengthen the notion that nodal expression is not allowed in the presence of functional Hbox12 in the same cells, these results highlight a critical role of histone deacetylases in regulating the spatial expression of hbox12.
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页数：16
相关论文
共 70 条
[1]   HISTONE ACETYLATION INFLUENCES BOTH GENE-EXPRESSION AND DEVELOPMENT OF XENOPUS-LAEVIS [J].
ALMOUZNI, G ;
KHOCHBIN, S ;
DIMITROV, S ;
WOLFFE, AP .
DEVELOPMENTAL BIOLOGY, 1994, 165 (02) :654-669
[2]   Patterning the sea urchin embryo: Gene regulatory networks, signaling pathways, and cellular interactions [J].
Angerer, LM ;
Angerer, RC .
CURRENT TOPICS IN DEVELOPMENTAL BIOLOGY, VOL 53, 2003, 53 :159-198
[3]  
Angerer LM, 2000, DEVELOPMENT, V127, P1105
[4]   The gene regulatory network basis of the "community effect," and analysis of a sea urchin embryo example [J].
Bolouri, Hamid ;
Davidson, Eric H. .
DEVELOPMENTAL BIOLOGY, 2010, 340 (02) :170-178
[5]   Chordin is required for neural but not axial development in sea urchin embryos [J].
Bradham, Cynthia A. ;
Oikonomou, Catherine ;
Kuehn, Alexander ;
Core, Amanda B. ;
Modell, Joshua W. ;
McClay, David R. ;
Poustka, Albert J. .
DEVELOPMENTAL BIOLOGY, 2009, 328 (02) :221-233
[6]   Impairing Otp homeodomain function in oral ectoderm cells affects skeletogenesis in sea urchin embryos [J].
Cavalieri, V ;
Spinelli, G ;
Di Bernardo, M .
DEVELOPMENTAL BIOLOGY, 2003, 262 (01) :107-118
[7]   cis-regulatory sequences driving the expression of the Hbox12 homeobox-containing gene in the presumptive aboral ectoderm territory of the Paracentrotus lividus sea urchin embryo [J].
Cavalieri, Vincenzo ;
Di Bernardo, Maria ;
Anello, Letizia ;
Spinelli, Giovanni .
DEVELOPMENTAL BIOLOGY, 2008, 321 (02) :455-469
[8]   Early asymmetric cues triggering the dorsal/ventral gene regulatory network of the sea urchin embryo [J].
Cavalieri, Vincenzo ;
Spinelli, Giovanni .
ELIFE, 2014, 3 :e04664
[9]   The Compass-like Locus, Exclusive to the Ambulacrarians, Encodes a Chromatin Insulator Binding Protein in the Sea Urchin Embryo [J].
Cavalieri, Vincenzo ;
Melfi, Raffaella ;
Spinelli, Giovanni .
PLOS GENETICS, 2013, 9 (09)
[10]   Specific expression of a TRIM-containing factor in ectoderm cells affects the skeletal morphogenetic program of the sea urchin embryo [J].
Cavalieri, Vincenzo ;
Guarcello, Rosa ;
Spinelli, Giovanni .
DEVELOPMENT, 2011, 138 (19) :4279-4290
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