Interaction of Ionic Liquid [bmin][CF3SO3] with Lysozyme Investigated by Two-Dimensional Fourier Transform Infrared Spectroscopy

被引：24

作者：

Du, Kun ^{[1
]}

Sun, Jian ^{[1
]}

Song, Xiaoqiang ^{[1
]}

Chen, Huaming ^{[1
]}

Feng, Wei ^{[1
]}

Ji, Peijun ^{[2
]}

机构：

[1] Beijing Univ Chem Technol, Dept Biochem Engn, Beijing Key Lab Bioproc, Beijing 100029, Peoples R China

[2] Beijing Univ Chem Technol, Dept Chem Engn, Beijing 100029, Peoples R China

来源：

ACS SUSTAINABLE CHEMISTRY & ENGINEERING | 2014年 / 2卷 / 06期

基金：

美国国家科学基金会;

关键词：

Ionic liquid; Lysozyme; Two-dimensional FTIR spectra; Interaction mechanism; EGG-WHITE LYSOZYME; LISTERIA-MONOCYTOGENES; PROTEIN-STRUCTURE; ORGANIC-COMPOUNDS; FOOD; FTIR; HEN; CONFORMATION; FLUORESCENCE; EXTRACTION;

D O I：

10.1021/sc500218e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The combination of ionic liquids with lysozyme has a potential application in food processing and analysis. In this work, at acid conditions, the interaction mechanism of the ionic liquid 1-butyl-3-methylimidazolium trifluoromethansulfonate with lysozyme has been investigated by two-dimensional Fourier transform infrared spectroscopy (FTIR). The residues and structures of lysozyme that have preferential interactions with the ionic liquid have been identified. The interaction mechanism can explain experimental results at acidic conditions where it was found that the presence of the ionic liquid is positively correlated to the enhanced enzymatic activity of the hen egg white lysozyme.

引用

页码：1420 / 1428

页数：9

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