Midzone organization restricts interpolar microtubule plus-end dynamics during spindle elongation

被引：34

作者：

Fridman, Vladimir ^{[1
]}

Gerson-Gurwitz, Adina ^{[2
]}

Movshovich, Natalia ^{[2
]}

Kupiec, Martin ^{[3
]}

Gheber, Larisa ^{[1
,2
]}

机构：

[1] Ben Gurion Univ Negev, Dept Clin Biochem, IL-84105 Beer Sheva, Israel

[2] Ben Gurion Univ Negev, Dept Chem, IL-84105 Beer Sheva, Israel

[3] Tel Aviv Univ, Dept Mol Microbiol & Biotechnol, IL-69978 Tel Aviv, Israel

来源：

EMBO REPORTS | 2009年 / 10卷 / 04期

基金：

以色列科学基金会;

关键词：

anaphase B; Ase1; Cin8; interpolar microtubule dynamics; midzone; MITOTIC SPINDLE; SACCHAROMYCES-CEREVISIAE; BUDDING YEAST; ANAPHASE-B; CHROMOSOME SEGREGATION; BIPOLAR KINESIN; CROSS-LINKS; MOTORS; SWITCH; CELLS;

D O I：

10.1038/embor.2009.7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

To study the dynamics of interpolar microtubules (iMTs) in Saccharomyces cerevisiae cells, we photobleached a considerable portion of the middle region of anaphase spindles in cells expressing tubulin-green fluorescent protein (GFP) and followed fluorescence recovery at the iMT plus-ends. We found that during anaphase, iMTs show phases of fast growth and shrinkage that are restricted to the iMT plus-ends. Our data indicate that iMT plus-end dynamics are regulated during mitosis, as fluorescence recovery was faster in intermediate anaphase (30 s) compared with long (100 s) and pre-anaphase (80 s) spindles. We also observed that deletion of Cin8, a microtubule-crosslinking kinesin-5 motor protein, reduced the recovery rate in anaphase spindles, indicating that Cin8 contributes to the destabilization of iMT plus-ends. Finally, we show that in cells lacking the midzone organizing protein Ase1, iMTs are highly dynamic and are exchangeable throughout most of their length, indicating that midzone organization is essential for restricting iMT dynamics.

引用

页码：387 / 393

页数：7

共 31 条

[1] Going mobile: Microtubule motors and chromosome segregation [J].