Association mapping of stem rust race TTKSK resistance in US barley breeding germplasm

被引：31

作者：

Zhou, H. ^{[1
]}

Steffenson, B. J. ^{[1
]}

Muehlbauer, Gary ^{[2
,3
]}

Wanyera, Ruth ^{[4
]}

Njau, Peter ^{[4
]}

Ndeda, Sylvester ^{[5
]}

机构：

[1] Univ Minnesota, Dept Plant Pathol, St Paul, MN 55108 USA

[2] Univ Minnesota, Dept Agron & Plant Genet, St Paul, MN USA

[3] Univ Minnesota, Dept Plant Biol, St Paul, MN USA

[4] Kenya Agr Res Inst, Njoro, Kenya

[5] East African Maltings Ltd, Molo, Kenya

来源：

THEORETICAL AND APPLIED GENETICS | 2014年 / 127卷 / 06期

基金：

美国食品与农业研究所;

关键词：

F-SP TRITICI; QUANTITATIVE TRAIT LOCI; LINKAGE DISEQUILIBRIUM; POPULATION-STRUCTURE; DURABLE RESISTANCE; SP SECALIS; WHEAT; INHERITANCE; GENETICS; PROTEIN;

D O I：

10.1007/s00122-014-2297-8

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Loci conferring resistance to the highly virulent African stem rust race TTKSK were identified in advanced barley breeding germplasm and positioned to chromosomes 5H and 7H using an association mapping approach. African races of the stem rust pathogen (Puccinia graminis f. sp. tritici) are a serious threat to barley production worldwide because of their wide virulence. To discover and characterize resistance to African stem rust race TTKSK in US barley breeding germplasm, over 3,000 lines/cultivars were assessed for resistance at the seedling stage in the greenhouse and also the adult plant stage in the field in Kenya. Only 12 (0.3 %) and 64 (2.1 %) lines exhibited a resistance level comparable to the resistant control at the seedling and adult plant stage, respectively. To map quantitative trait loci (QTL) for resistance to race TTKSK, an association mapping approach was conducted, utilizing 3,072 single nucleotide polymorphism (SNP) markers. At the seedling stage, two neighboring SNP markers (0.8 cM apart) on chromosome 7H (11_21491 and 12_30528) were found significantly associated with resistance. The most significant one found was 12_30528; thus, the resistance QTL was named Rpg-qtl-7H-12_30528. At the adult plant stage, two SNP markers on chromosome 5H (11_11355 and 12_31427) were found significantly associated with resistance. This resistance QTL was named Rpg-qtl-5H-11_11355 for the most significant marker identified. Adult plant resistance is of paramount importance for stem rust. The marker associated with Rpg-qtl-5H-11_11355 for adult plant resistance explained only a small portion of the phenotypic variation (0.02); however, this QTL reduced disease severity up to 55.0 % under low disease pressure and up to 21.1 % under heavy disease pressure. SNP marker 11_11355 will be valuable for marker-assisted selection of adult plant stem rust resistance in barley breeding.

引用

页码：1293 / 1304

页数：12

共 65 条

[1] CONTROLLING THE FALSE DISCOVERY RATE - A PRACTICAL AND POWERFUL APPROACH TO MULTIPLE TESTING [J].

BENJAMINI, Y ;

HOCHBERG, Y .

JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES B-STATISTICAL METHODOLOGY, 1995, 57 (01) :289-300

[2] Association mapping of kernel size and milling quality in wheat (Triticum aestivum L.) cultivars [J].

Breseghello, F ;

Sorrells, ME .

GENETICS, 2006, 172 (02) :1165-1177

[3] The stem rust resistance gene Rpg5 encodes a protein with nucleotide-binding-site, leucine-rich, and protein kinase domains [J].

Brueggeman, R. ;

Druka, A. ;

Nirmala, J. ;

Cavileer, T. ;

Drader, T. ;

Rostoks, N. ;

Mirlohi, A. ;

Bennypaul, H. ;

Gill, U. ;

Kudrna, D. ;

Whitelaw, C. ;

Kilian, A. ;

Han, F. ;

Sun, Y. ;

Gill, K. ;

Steffenson, B. ;

Kleinhofs, A. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (39) :14970-14975

[4] Development and implementation of high-throughput SNP genotyping in barley [J].

Close, Timothy J. ;

Bhat, Prasanna R. ;

Lonardi, Stefano ;

Wu, Yonghui ;

Rostoks, Nils ;

Ramsay, Luke ;

Druka, Arnis ;

Stein, Nils ;

Svensson, Jan T. ;

Wanamaker, Steve ;

Bozdag, Serdar ;

Roose, Mikeal L. ;

Moscou, Matthew J. ;

Chao, Shiaoman ;

Varshney, Rajeev K. ;

Szuecs, Peter ;

Sato, Kazuhiro ;

Hayes, Patrick M. ;

Matthews, David E. ;

Kleinhofs, Andris ;

Muehlbauer, Gary J. ;

DeYoung, Joseph ;

Marshall, David F. ;

Madishetty, Kavitha ;

Fenton, Raymond D. ;

Condamine, Pascal ;

Graner, Andreas ;

Waugh, Robbie .

BMC GENOMICS, 2009, 10

[5] Genome-wide association mapping to candidate polymorphism resolution in the unsequenced barley genome [J].

Cockram, James ;

White, Jon ;

Zuluaga, Diana L. ;

Smith, David ;

Comadran, Jordi ;

Macaulay, Malcolm ;

Luo, Zewei ;

Kearsey, Mike J. ;

Werner, Peter ;

Harrap, David ;

Tapsell, Chris ;

Liu, Hui ;

Hedley, Peter E. ;

Stein, Nils ;

Schulte, Daniela ;

Steuernagel, Burkhard ;

Marshall, David F. ;

Thomas, William T. B. ;

Ramsay, Luke ;

Mackay, Ian ;

Balding, David J. ;

Waugh, Robbie ;

O'Sullivan, Donal M. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2010, 107 (50) :21611-21616

[6] Natural variation in a homolog of Antirrhinum CENTRORADIALIS contributed to spring growth habit and environmental adaptation in cultivated barley [J].

Comadran, Jordi ;

Kilian, Benjamin ;

Russell, Joanne ;

Ramsay, Luke ;

Stein, Nils ;

Ganal, Martin ;

Shaw, Paul ;

Bayer, Micha ;

Thomas, William ;

Marshall, David ;

Hedley, Pete ;

Tondelli, Alessandro ;

Pecchioni, Nicola ;

Francia, Enrico ;

Korzun, Viktor ;

Walther, Alexander ;

Waugh, Robbie .

NATURE GENETICS, 2012, 44 (12) :1388-1392

[7] Genome-wide SNPs and re-sequencing of growth habit and inflorescence genes in barley: implications for association mapping in germplasm arrays varying in size and structure [J].

Cuesta-Marcos, Alfonso ;

Szucs, Peter ;

Close, Timothy J. ;

Filichkin, Tanya ;

Muehlbauer, Gary J. ;

Smith, Kevin P. ;

Hayes, Patrick M. .

BMC GENOMICS, 2010, 11

[8] Plant pathogens and integrated defence responses to infection [J].

Dangl, JL ;

Jones, JDG .

NATURE, 2001, 411 (6839) :826-833

[9] INOCULUM PRESSURE AND THE DEVELOPMENT OF STEM RUST EPIDEMICS IN BARLEY [J].

DILLMACKY, R ;

REES, RG ;

PLATZ, GJ .

AUSTRALIAN JOURNAL OF AGRICULTURAL RESEARCH, 1991, 42 (05) :769-777

[10] Mapping and analysis of quantitative trait loci in experimental populations [J].

Doerge, RW .

NATURE REVIEWS GENETICS, 2002, 3 (01) :43-52

← 1 2 3 4 5 6 7 →