Performance of the ImmunoCard STAT!: E. coli O157:H7 test for detection of Escherichia coli O157:H7 in stools

被引：13
作者：
Mackenzie, A
Orrbine, E
Hyde, L
Benoit, M
Chan, F
Park, C
Alverson, J
Lembke, A
Hoban, D
Kennedy, W
机构：
[1] Childrens Hosp Eastern Ontario, Res Inst, Child & Youth Clin Trials Network, Ottawa, ON K1H 8L1, Canada
[2] Hlth Sci Ctr, Winnipeg, MB, Canada
[3] Isaak Walton Killam Hosp, Halifax, NS, Canada
[4] INOVA Fairfax Hosp, Falls Church, VA USA
[5] Scottish Rite Childrens Hosp, Atlanta, GA USA
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2000年 / 38卷 / 05期
关键词：
D O I：
10.1128/JCM.38.5.1866-1868.2000
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
ImmunoCard STAT! E, coli O157:H7 (Meridian Diagnostics, Inc, Cincinnati, Ohio) is a novel rapid (10-min) test for the presence of Escherichia coli O157:H7 in stools. The test may be performed either directly on stool specimens or on an overnight broth culture of stool. In a multicenter prospective study, 14 of 14 specimens positive by culture for E. coli O157:H7 were positive by the ImmunoCard STAT! O157:H7 test, and there were no false positives from 263 culture-negative specimens. In a retrospective study, the test was positive in 339 (81%) of 417 stored culture-positive specimens and the specificity was 95% (98 of 103 specimens). No false positives were associated with alternate stool pathogens. The ImmunoCard STAT! O157:H7 Lest has high sensitivity and specificity.
引用
收藏
页码：1866 / 1868
页数：3
相关论文
共 11 条
[1]  
BROTMAN M, 1995, GASTROENTEROLOGY, V108, P1923, DOI 10.1016/0016-5085(95)90159-0
[2]   THE EPIDEMIOLOGY OF INFECTIONS CAUSED BY ESCHERICHIA-COLI O157-H7, OTHER ENTEROHEMORRHAGIC ESCHERICHIA-COLI, AND THE ASSOCIATED HEMOLYTIC UREMIC SYNDROME [J].
GRIFFIN, PM ;
TAUXE, RV .
EPIDEMIOLOGIC REVIEWS, 1991, 13 :60-98
[3]  
Griffin PM, 1998, ESCHERICHIA COLI O15, P15
[4]   Evaluation of a microplate latex agglutination method (Verotox-F assay) for detecting and characterizing verotoxins (Shiga toxins) in Escherichia coli [J].
Karmali, MA ;
Petric, M ;
Bielaszewska, M .
JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (02) :396-399
[5]   Evaluation of the premier EHEC assay for detection of shiga toxin-producing Escherichia coli [J].
Kehl, KS ;
Havens, P ;
Behnke, CE ;
Acheson, DWK .
JOURNAL OF CLINICAL MICROBIOLOGY, 1997, 35 (08) :2051-2054
[6]   HEMOLYTIC-UREMIC SYNDROME - AN ANALYSIS OF THE NATURAL-HISTORY AND PROGNOSTIC FEATURES [J].
LOIRAT, C ;
SONSINO, E ;
MORENO, AV ;
PILLION, G ;
MERCIER, JC ;
BEAUFILS, F ;
MATHIEU, H .
ACTA PAEDIATRICA SCANDINAVICA, 1984, 73 (04) :505-514
[7]   Sensitivities and specificities of premier E-coli O157 and premier EHEC enzyme immunoassays for diagnosis of infection with verotoxin (shiga-like toxin)-producing Escherichia coli [J].
MacKenzie, AMR ;
Lebel, P ;
Orrbine, E ;
Rowe, PC ;
Hyde, L ;
Chan, F ;
Johnson, W ;
McLaine, PN .
JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (06) :1608-1611
[8]   SPORADIC CASES OF HEMORRHAGIC COLITIS ASSOCIATED WITH ESCHERICHIA-COLI O157-H7 - CLINICAL, EPIDEMIOLOGIC, AND BACTERIOLOGIC FEATURES [J].
PAI, CH ;
GORDON, R ;
SIMS, HV ;
BRYAN, LE .
ANNALS OF INTERNAL MEDICINE, 1984, 101 (06) :738-742
[9]   Isolation of Shiga-like toxin producing Escherichia coli (O157 and non-O157) in a community hospital [J].
Park, CH ;
Gates, KM ;
Vandel, NM ;
Hixon, DL .
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 1996, 26 (02) :69-72
[10]   Risk of hemolytic uremic syndrome after sporadic Escherichia coli O157:H7 infection:: Results of a Canadian collaborative study [J].
Rowe, PC ;
Orrbine, E ;
Lior, H ;
Wells, GA ;
Yetisir, E ;
Clulow, M ;
McLaine, PN .
JOURNAL OF PEDIATRICS, 1998, 132 (05) :777-782
12