An African Salmonella Typhimurium ST313 sublineage with extensive drug-resistance and signatures of host adaptation

被引：86

作者：

Van Puyvelde, Sandra ^{[1
,2
,3
]}

Pickard, Derek ^{[2
,4
]}

Vandelannoote, Koen ^{[1
]}

Heinz, Eva ^{[2
,5
]}

Barbe, Barbara ^{[6
]}

de Block, Tessa ^{[1
]}

Clare, Simon ^{[2
]}

Coomber, Eve L. ^{[2
]}

Harcourt, Katherine ^{[2
]}

Sridhar, Sushmita ^{[2
,4
]}

Lees, Emily A. ^{[2
,4
]}

Wheeler, Nicole E. ^{[7
]}

Klemm, Elizabeth J. ^{[2
]}

Kuijpers, Laura ^{[6
,8
]}

Kalonji, Lisette Mbuyi ^{[9
,10
]}

Phoba, Marie-France ^{[9
,10
]}

Falay, Dadi ^{[11
]}

Ngbonda, Dauly ^{[11
]}

Lunguya, Octavie ^{[9
,10
]}

Jacobs, Jan ^{[6
,8
]}

Dougan, Gordon ^{[2
,4
]}

Deborggraeve, Stijn ^{[1
]}

机构：

[1] Inst Trop Med, Dept Biomed Sci, Natl Str 155, B-2000 Antwerp, Belgium

[2] Wellcome Sanger Inst, Wellcome Genome Campus, Cambridge CB10 1SA, England

[3] Univ Antwerp, Lab Med Microbiol, Vaccine & Infect Dis Inst, Antwerp, Belgium

[4] Univ Cambridge, Dept Med, Addenbrookes Hosp, Cambridge CB2 0SP, England

[5] Univ Liverpool Liverpool Sch Trop Med, Dept Vector Biol, Pembroke Pl, Liverpool L3 5QA, Merseyside, England

[6] Inst Trop Med, Dept Clin Sci, Natl Str 155, B-2000 Antwerp, Belgium

[7] Wellcome Sanger Inst, Ctr Genom Pathogen Surveillance, Wellcome Genome Campus, Cambridge CB10 1SA, England

[8] Katholieke Univ Leuven, Dept Microbiol & Immunol, Herestr 49 Box 1030, B-3000 Leuven, Belgium

[9] Natl Inst Biomed Res, Dept Microbiol, Av Democratie 5345, Kinshasa, DEM REP CONGO

[10] Univ Hosp Kinshasa, Dept Microbiol, Kinshasa, DEM REP CONGO

[11] Univ Hosp Kisangani, Dept Pediat, Ave Munyororo C Makiso,BP 2012, Kisangani, DEM REP CONGO

来源：

NATURE COMMUNICATIONS | 2019年 / 10卷 / 1期

基金：

英国惠康基金;

关键词：

SPECTRUM BETA-LACTAMASE; NONTYPHOIDAL SALMONELLA; ENTERICA; INFECTIONS; SEQUENCE; DISEASE; EPIDEMIOLOGY; ANNOTATION; NUCLEOTIDE; DISCOVERY;

D O I：

10.1038/s41467-019-11844-z

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Bloodstream infections by Salmonella enterica serovar Typhimurium constitute a major health burden in sub-Saharan Africa (SSA). These invasive non-typhoidal (iNTS) infections are dominated by isolates of the antibiotic resistance-associated sequence type (ST) 313. Here, we report emergence of ST313 sublineage II.1 in the Democratic Republic of the Congo. Sublineage II.1 exhibits extensive drug resistance, involving a combination of multidrug resistance, extended spectrum beta-lactamase production and azithromycin resistance. ST313 lineage II.1 isolates harbour an IncHI2 plasmid we name pSTm-ST313-II.1, with one isolate also exhibiting decreased ciprofloxacin susceptibility. Whole genome sequencing reveals that ST313 II.1 isolates have accumulated genetic signatures potentially associated with altered pathogenicity and host adaptation, related to changes observed in biofilm formation and metabolic capacity. Sublineage II.1 emerged at the beginning of the 21st century and is involved in on-going outbreaks. Our data provide evidence of further evolution within the ST313 clade associated with iNTS in SSA.

引用

页数：12

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