Genetic Reagents for Making Split-GAL4 Lines in Drosophila

被引：111

作者：

Dionne, Heather ^{[1
]}

Hibbard, Karen L. ^{[1
]}

Cavallaro, Amanda ^{[1
]}

Kao, Jui-Chun ^{[1
]}

Rubin, Gerald M. ^{[1
]}

机构：

[1] Howard Hughes Med Inst, Janelia Res Campus, Ashburn, VA 20147 USA

来源：

GENETICS | 2018年 / 209卷 / 01期

关键词：

gene expression; enhancer; genetic intersection; neuron; cell type; EXPRESSION; SYSTEM; TOOLS; INTEGRASE;

D O I：

10.1534/genetics.118.300682

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The ability to reproducibly target expression of transgenes to small, defined subsets of cells is a key experimental tool for understanding many biological processes. The Drosophila nervous system contains thousands of distinct cell types and it has generally not been possible to limit expression to one or a few cell types when using a single segment of genomic DNA as an enhancer to drive expression. Intersectional methods, in which expression of the transgene only occurs where two different enhancers overlap in their expression patterns, can be used to achieve the desired specificity. This report describes a set of over 2800 transgenic lines for use with the split-GAL4 intersectional method.

引用

页码：31 / 35

页数：5

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