Effect of atorvastatin on expression of IL-10 and TNF-alpha mRNA in myocardial ischemia-reperfusion injury in rats

被引：27

作者：

Sun, Yan-Ming ^{[2
]}

Tian, Ye ^{[1
,3
]}

Li, Xiang ^{[1
]}

Liu, Yuan-Yuan ^{[1
]}

Wang, Lan-Feng ^{[2
]}

Li, Jia ^{[2
]}

Li, Zhu-Qin ^{[2
]}

Pan, Wei ^{[2
]}

机构：

[1] Harbin Med Coll, Affiliated Hosp 2, Dept Cardiol, Harbin 150086, Peoples R China

[2] Harbin Med Coll, Affiliated Hosp 1, Dept Cardiac Care Unit, Harbin 150086, Peoples R China

[3] Harbin Med Coll, Dept Pathophysiol, Harbin 150086, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 382卷 / 02期

关键词：

Myocardial ischemia-reperfusion; IL-10; 3-Hydroxy-3-methylglutaryl coenzyme A; reductse inhibitor; TNF-alpha; NECROSIS-FACTOR-ALPHA; NITRIC-OXIDE; HEART-DISEASE; T-CELLS; ATHEROSCLEROSIS; INTERLEUKIN-10; ROSUVASTATIN; ACTIVATION; INFARCTION; MONOCYTES;

D O I：

10.1016/j.bbrc.2009.03.019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Myocardial ischemia and reperfusion (MI/R) is associated with an intense inflammatory reaction, which may lead to myocyte injury. Because statins protect the myocardium against ischemia-reperfusion injury via a mechanism unrelated to cholesterol-lowering, we hypothesized that the protective effect of statins was related to the expression of TNF-alpha (TNF-alpha) and interleukin-10 (IL-10) mRNA. Seventy-two rats were randomly divided into three groups as follows: sham, I/R and I/R + atorvastatin. Atorvastatin (20 mg kg(-1) day(-1)) treatment was administered daily via oral gavage to rats for 2, 7 or 14 days. Ischemia was induced via a 30-min coronary occlusion. Reperfusion was allowed until 2, 7 or 14 days while atorvastatin treatment continued. We measured infarct size, hemodynamics and the plasma levels and the mRNA expression of TNF-alpha and IL-10 in the three groups. We demonstrated that the up-regulation of expression of both TNF-alpha mRNA and IL-10 mRNA was associated the increased plasma levels of TNF-alpha and IL-10 in the ischemic and reperfused myocardium compared with that in the sham group (P<0.01). Atorvastatin treatment prevented ischemia-reperfusion-induced up-regulation of both TNF-alpha and IL-10 mRNA, and improved left ventricular function (P<0.01). Our findings suggested that atorvastatin may attenuate MI/R and better recovery of left ventricle function following ischemia and reperfusion and IL-10 was not directly likely involved in this protective mechanism. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：336 / 340

页数：5

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