IGF2 Methylation Is Associated with Lipid Profile in Obese Children

被引:22
作者
Deodati, Annalisa [1 ]
Inzaghi, Elena [2 ]
Liguori, Alice [1 ]
Puglianiello, Antonella [1 ]
Germani, Daniela [1 ]
Brufani, Claudia [2 ]
Fintini, Danilo [3 ]
Cappa, Marco [2 ]
Barbetti, Fabrizio [4 ]
Cianfarani, Stefano [2 ,5 ]
机构
[1] Univ Roma Tor Vergata, Dept Syst Med, Rome, Italy
[2] Bambino Gesu Pediat Hosp, Endocrinol Unit, IT-00165 Rome, Italy
[3] Bambino Gesu Pediat Hosp, Cardioresp & Sport Med Unit, IT-00165 Rome, Italy
[4] Tor Vergata Univ Hosp, Lab Monogen Diabet, Bambino Gesu Childrens Hosp, Dept Lab Med, Rome, Italy
[5] Karolinska Inst, Dept Womens & Childrens Hlth, Stockholm, Sweden
来源
HORMONE RESEARCH IN PAEDIATRICS | 2013年 / 79卷 / 06期
关键词
IGF2; Obesity; Dyslipidemia; BETA-CELL FUNCTION; GROWTH-FACTOR-II; PRENATAL EXPOSURE; INSULIN SENSITIVITY; GLUCOSE-TOLERANCE; GENE-EXPRESSION; DUTCH FAMINE; WEIGHT; RECEPTOR; RISK;
D O I
10.1159/000351707
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Aim: Our aim was to investigate the relationships between the degree of IGF2 methylation and the metabolic status in obese children and adolescents. Subjects and Methods: Eighty-five obese subjects aged 11.6 +/- 2.1 years were studied. Anthropometry, metabolic parameters, blood pressure and body composition were assessed. DNA methylation analysis was performed by restriction enzyme digestion assay. The study population was subdivided into two groups according to the percentage of IGF2 cytidine-guanosine (CpG) island methylation. Results: Twenty-two subjects showed intermediate methylation (a percentage of CpG site methylation comprised between 10 and 60%), 56 were hypomethylated (percentage of methylation lower than 10%), and only 1 showed a high rate of hypermethylation (percentage of methylation above 60%). Children with intermediate methylation showed significantly higher levels of triglycerides (107.6 +/- 41.99 vs. 76.6 +/- 30.18 mg/dl, p < 0.005) and a higher triglyceride/high-density lipoprotein-cholesterol ratio (2.23 +/- 0.98 vs. 1.79 +/- 0.98, p < 0.02) compared with hypomethylated children. Conclusions: These preliminary findings show for the first time a relationship between IGF2 methylation pattern and lipid profile in obese children. Although the correlation does not imply causation, if our findings are confirmed in further studies, IGF2 methylation might represent an epigenetic marker of metabolic risk. Copyright (C) 2013 S. Karger AG, Basel
引用
收藏
页码:361 / 367
页数:7
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