Expression system for production of bioactive compounds, recombinant human adiponectin, in the silk glands of transgenic silkworms

被引：8

作者：

Shin, Seulmee ^{[1
]}

Kim, Bong-Yoon ^{[2
]}

Jeon, Hyung-Yook ^{[3
]}

Lee, Aeri ^{[1
]}

Lee, Sungwon ^{[1
]}

Sung, Seung-Hyun ^{[3
]}

Park, Chan-Su ^{[4
]}

Lee, Chong-Kil ^{[4
]}

Kong, Hyunseok ^{[1
]}

Song, Youngcheon ^{[1
]}

Kim, Kyungjae ^{[1
]}

机构：

[1] Sahmyook Univ, Coll Pharm, Seoul 139742, South Korea

[2] B2Bio Co Ltd, Seoul 139230, South Korea

[3] AlkaGENE Co Ltd, Songnam 463760, Kyeonggi Do, South Korea

[4] Chungbuk Natl Univ, Coll Pharm, Cheongju 361763, South Korea

来源：

ARCHIVES OF PHARMACAL RESEARCH | 2014年 / 37卷 / 05期

关键词：

Adiponectin; Bombyx mori; Transgenic silkworms; Silk glands; INSULIN-RESISTANCE; PROTEIN; ADIPOSE; EXCISION;

D O I：

10.1007/s12272-013-0298-9

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Adiponectin is an adipocyte hormone involved in glucose and lipid metabolism. The aim of this study was to develop a human adiponectin expression system in transgenic silkworm using a human adiponectin expression vector. The silk gland of the silkworm is a highly specialized organ that has the wonderful ability to synthesize and secrete silk protein. To express human adiponectin in the silk gland of transgenic silkworm, targeting vectors pB-A3-adiponectin-IRES-RFP and pB-Ser1-adiponectin-IRES-RFP were constructed and then introduced into the silkworm pupa. The transgenic silkworms were verified by PCR and then generated. The level of adiponectin in the transgenic silkworm was 6-10 ng/50 mg of freeze-dried powder, and western blotting using an antibody against human adiponectin demonstrated a specific band with a molecular weight of 30 kDa in the silkworm. These results showed that human adiponectin introduced into the silkworm genome was expressed successfully on a large-scale.

引用

页码：645 / 651

页数：7

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