Human amylin stimulates inflammatory cytokine secretion from human glioma cells

被引:17
|
作者
Gitter, BD [1 ]
Cox, LM [1 ]
Carlson, CD [1 ]
May, PC [1 ]
机构
[1] Eli Lilly & Co, Lilly Res Labs, Neurosci Dis Res Div, Lilly Corp Ctr, Indianapolis, IN 46285 USA
关键词
amylin; Alzheimer's disease; neuroinflammation;
D O I
10.1159/000026432
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Chronic neurodegeneration in the brains of Alzheimer's disease (AD) patients may be mediated, at least in part, by the ability of amyloid beta (A beta) to exacerbate inflammatory pathways in a conformation-dependent manner. In this regard, we previously reported that the A beta-peptide-mediated potentiation of inflammatory cytokine secretion from interleukin-1 beta (IL-1 beta)-stimulated human astrocytoma cells was conformation dependent. Other amyloidogenic peptides, such as human amylin, which display similar conformation-dependent neurotoxic effects, may also elicit inflammatory cytokine secretion from glial cells. To test this hypothesis, we compared human and rat amylin for the effects on cytokine production in U-373 MG human astrocytoma cells. Human amylin alone stimulated U-373 MG cells to secrete IL-6 and IL-8 in a concentration-dependent manner with maximum effects seen at 10-25 mu M peptide. In addition, human amylin markedly potentiated IL-1 beta-stimulated cytokine production with a similar concentration dependence. In contrast, nonamyloidogenic rat amylin modestly stimulated cytokine secretion, either alone or combined with IL-1 beta. Aging human amylin resulted in diminished cytokine secretion, probably due to the formation of large, less active aggregates. In agreement with our previous studies using A beta, extracellular Ca2+ was necessary for human amylin stimulation of cytokine secretion. Our data suggest that amyloidogenic peptides promote cytokine secretion through similar beta-sheeted secondary-structure- and extracellular-Ca2+-dependent mechanism s. Copyright (C) 2000 S. Karger AG, Basel.
引用
收藏
页码:147 / 152
页数:6
相关论文
共 50 条
  • [41] Oxidative Stress Induces Parallel Autophagy and Mitochondria Dysfunction in Human Glioma U251 Cells
    Zhang, Hongyu
    Kong, Xiaoxia
    Kang, Jinsong
    Su, Jing
    Li, Yang
    Zhong, Jiateng
    Sun, Liankun
    TOXICOLOGICAL SCIENCES, 2009, 110 (02) : 376 - 388
  • [42] Amyloid β peptide promotes differentiation of pro-inflammatory human myeloid dendritic cells
    Ciaramella, Antonio
    Sanarico, Nunzia
    Bizzoni, Federica
    Moro, Maria Luisa
    Salani, Francesca
    Scapighati, Giuseppe
    Spalletta, Gianfranco
    Caltagirone, Carlo
    Bossu, Paola
    NEUROBIOLOGY OF AGING, 2009, 30 (02) : 210 - 221
  • [43] Inhibitory Effect of Punicalagin on Inflammatory and Angiogenic Activation of Human Umbilical Vein Endothelial Cells
    Liu, Wei
    Ou, Yanghui
    Yang, Yumeng
    Zhang, Xuemei
    Huang, Liqi
    Wang, Xiaohua
    Wu, Buling
    Huang, Mingcheng
    FRONTIERS IN PHARMACOLOGY, 2021, 12
  • [44] Activation of activating transcription factor 2 by p38 MAP kinase during apoptosis induced by human amylin in cultured pancreatic β-cells
    Zhang, Shaoping
    Liu, Hong
    Liu, Junxi
    Tse, Cynthia A.
    Dragunow, Michael
    Cooper, Garth J. S.
    FEBS JOURNAL, 2006, 273 (16) : 3779 - 3791
  • [45] The Anti-Inflammatory Effects of Lipoxygenase and Cyclo-Oxygenase Inhibitors in Inflammation-Induced Human Fetal Glia Cells and the Aβ Degradation Capacity of Human Fetal Astrocytes in an Ex vivo Assay
    Pihlaja, Rea
    Haaparanta-Solin, Merja
    Rinne, Juha O.
    FRONTIERS IN NEUROSCIENCE, 2017, 11
  • [46] Lipoprotein lipase and phospholipid transfer protein overexpression in human glioma cells and their effect on cell growth, apoptosis, and migration
    Dong, Weijiang
    Gong, Huilin
    Zhang, Guanjun
    Vuletic, Simona
    Albers, John
    Zhang, Jiaojiao
    Liang, Hua
    Sui, Yanxia
    Zheng, Jin
    ACTA BIOCHIMICA ET BIOPHYSICA SINICA, 2017, 49 (01) : 62 - 73
  • [47] 24S-hydroxycholesterol induces inflammatory gene expression in primary human neural cells
    Alexandrov, P
    Cui, JG
    Zhao, YH
    Lukiw, WJ
    NEUROREPORT, 2005, 16 (09) : 909 - 913
  • [48] THE EFFECTS OF AMYLIN ON INSULIN-SECRETION FROM RIN M5F CELLS AND GLYCOGEN-SYNTHESIS AND LIPOGENESIS IN RAT PRIMARY CULTURED-HEPATOCYTES
    SUZUKI, S
    MURAKAMI, M
    ABE, S
    SATOH, Y
    SHINTANI, S
    ISHIZUKA, J
    SUZUKI, K
    THOMPSON, JC
    TOYOTA, T
    DIABETES RESEARCH AND CLINICAL PRACTICE, 1992, 15 (01) : 77 - 84
  • [49] A fluorogenic-inhibitor-based probe for profiling and imaging of monoamine oxidase A in live human glioma cells and clinical tissues
    Fang, Haixiao
    Li, Panpan
    Shen, Congzhen
    Tang, Fang
    Ding, Aixiang
    Bai, Hua
    Peng, Bo
    Yang, Xuekang
    Li, Zhengqiu
    Huang, Kai
    Pan, Sijun
    Li, Lin
    Huang, Wei
    SCIENCE CHINA-CHEMISTRY, 2023, 66 (07) : 2053 - 2061
  • [50] Dimethyl fumarate activates the prostaglandin EP2 receptor and stimulates cAMP signaling in human peripheral blood mononuclear cells
    Fiedler, Sarah E.
    Kerns, Amelia R.
    Tsang, Catherine
    Tsang, Vivian
    Bourdette, Dennis
    Salinthone, Sonemany
    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2016, 475 (01) : 19 - 24