Specific 14-3-3 isoform detection and immunolocalization in prion diseases

被引：31
作者：
Baxter, HC
Fraser, JR
Liu, WG
Forster, JL
Clokie, S
Steinacker, P
Otto, M
Bahn, E
Wiltfang, J
Aitken, A
机构：
[1] Univ Edinburgh, Dept Biomed Sci, Edinburgh EH8 9XD, Midlothian, Scotland
[2] Inst Anim Hlth, Neuropathogenesis Unit, Edinburgh EH9 3JF, Midlothian, Scotland
[3] Univ Gottingen, Dept Neurol, D-37075 Gottingen, Germany
[4] Univ Gottingen, Dept Neuropathol, D-37075 Gottingen, Germany
[5] Univ Gottingen, Dept Psychiat, D-37075 Gottingen, Germany
来源：
BIOCHEMICAL SOCIETY TRANSACTIONS | 2002年 / 30卷
关键词：
CSF; ELISA; immunocytochemistry; neurodegeneration;
D O I：
10.1042/bst0300387
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
14-3-3 proteins are involved in signalling processes in neuronal cells. Using isoform-specific antibodies we have examined the variation in 14-3-3 isoform neurolocation in normal and scrapie-infected murine brain and show that in defined areas of the brain there are significant changes associated with the pathology of the disease process. The appearance of 14-3-3 proteins in the cerebrospinal fluid (CSF) is a consequence of neuronal disease and the detection of specific isoforms of the 14-3-3 proteins in the CSF is characteristic of some neurodegenerative diseases. In this study, monitoring specifically for the gamma 14-3-3 isoform in the CSF by both Western-blot analysis and ELISA we can show a level of correlation between the assays.
引用
收藏
页码：387 / 391
页数：5
相关论文
共 28 条
[11]  
2-A
[12]   Neurofibrillary tangles of Alzheimer's disease brains contain 14-3-3 proteins [J].
Layfield, R ;
Fergusson, J ;
Aitken, A ;
Lowe, J ;
Landon, M ;
Mayer, RJ .
NEUROSCIENCE LETTERS, 1996, 209 (01) :57-60
[13]   The assay development of a molecular marker for transmissible spongiform encephalopathies [J].
Lee, KH ;
Harrington, MG .
ELECTROPHORESIS, 1997, 18 (3-4) :502-506
[14]  
MARTIN H, 1994, J NEUROCHEM, V63, P2259
[15]   Interaction of 14-3-3 with signaling proteins is mediated by the recognition of phosphoserine [J].
Muslin, AJ ;
Tanner, JW ;
Allen, PM ;
Shaw, AS .
CELL, 1996, 84 (06) :889-897
[16]   S-100 protein concentration in the cerebrospinal fluid of patients with Creutzfeldt-Jakob disease [J].
Otto, M ;
Stein, H ;
Szudra, A ;
Zerr, I ;
Bodemer, M ;
Gefeller, O ;
Poser, S ;
Kretzschmar, HA ;
Mader, M ;
Weber, T .
JOURNAL OF NEUROLOGY, 1997, 244 (09) :566-570
[17]   Elevated levels of tau-protein in cerebrospinal fluid of patients with Creutzfeldt-Jakob disease [J].
Otto, M ;
Wiltfang, J ;
Tumani, H ;
Zerr, I ;
Lantsch, M ;
Kornhuber, J ;
Weber, T ;
Kretzschmar, HA ;
Poser, S .
NEUROSCIENCE LETTERS, 1997, 225 (03) :210-212
[18]   NOVEL PROTEINACEOUS INFECTIOUS PARTICLES CAUSE SCRAPIE [J].
PRUSINER, SB .
SCIENCE, 1982, 216 (4542) :136-144
[19]   The 14-3-3 protein detectable in the cerebrospinal fluid of patients with prion-unrelated neurological diseases is expressed constitutively in neurons and glial cells in culture [J].
Satoh, J ;
Kurohara, K ;
Yukitake, M ;
Kuroda, Y .
EUROPEAN NEUROLOGY, 1999, 41 (04) :216-225
[20]   Increased levels of ε and γ isoforms of 14-3-3 proteins in cerebrospinal fluid in patients with Creutzfeldt-Jakob disease [J].
Takahashi, H ;
Iwata, T ;
Kitagawa, Y ;
Takahashi, RH ;
Sato, Y ;
Wakabayashi, H ;
Takashima, M ;
Kido, H ;
Nagashima, K ;
Kenney, K ;
Gibbs, CJ ;
Kurata, T .
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY, 1999, 6 (06) :983-985
123