Effect of Tetramethylpyrazine on Atherosclerosis and SCAP/SREBP-1c Signaling Pathway in ApoE-/- Mice Fed with a High-Fat Diet

被引：16
作者：
Zhang, Ying [1 ]
Ren, Pan [2 ]
Kang, Qunfu [2 ,3 ]
Liu, Weihong [2 ]
Li, Sinai [2 ]
Li, Ping [2 ]
Liu, Hongxu [4 ]
Shang, Juju [4 ]
Zhang, Lei [2 ]
Gong, Yanbing [5 ]
Zhou, Mingxue [2 ]
机构：
[1] Capital Med Univ, Beijing Luhe Hosp, Dept TCM, Beijing 100149, Peoples R China
[2] Capital Med Univ, Beijing Inst Tradit Chinese Med, Beijing Hosp Tradit Chinese Med, Beijing 100010, Peoples R China
[3] Beijing Hosp Tradit Chinese Med, Shunyi Branch, Beijing 101300, Peoples R China
[4] Capital Med Univ, Beijing Hosp Tradit Chinese Med, Beijing 100010, Peoples R China
[5] Beijing Univ Chinese Med, Dongfang Hosp, Beijing 100078, Peoples R China
来源：
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE | 2017年 / 2017卷
基金：
中国国家自然科学基金; 北京市自然科学基金;
关键词：
REPERFUSION INJURY; LIGUSTRAZINE; PROTEIN; PAQR3; HOMEOSTASIS; MECHANISMS; EXPRESSION; ISCHEMIA; C57BL/6J; OBESITY;
D O I：
10.1155/2017/3121989
中图分类号：
R [医药、卫生];
学科分类号：
10 ;
摘要：
Lipid metabolism dysregulation plays a crucial role in the occurrence of atherosclerosis (As). SCAP/SREBP signaling is the main pathway for regulating lipid metabolism. Tetramethylpyrazine (TMP), a Traditional Chinese Medicine (TCM) for treating angina pectoris, has antiatherosclerotic effects and ameliorates blood lipids disturbance. However, its precise mechanism remains unclear. This study investigated the mechanism of TMP in ameliorating As in mice model. After six weeks of high-fat diet, 30 ApoE(-/-) mice were randomized (n = 10) and treated with Lipitor, TMP, or distilled water for six weeks. The serum blood lipids and insulin levels were measured. The expressions of PAQR3, Insig-1, SCAP, SREBP-1c, IRS-1, PI3K, Akt, and mTORC-1 in the adipose tissues were determined. The results showed that TMP could significantly decrease blood lipids levels, insulin, and corrected plaque area of the ApoE(-/-) mice as compared to the untreated mice (p < 0.05, p < 0.01). Moreover, TMP could significantly downregulate the expressions of SCAP, SREBP-1c, PAQR3, IRS-1, PI3K, Akt, and mTORC1 (p < 0.01). Thus, TMP may ameliorate lipid metabolism disorder and As by downregulating PAQR3 and inhibiting SCAP/SREBP-1c signaling pathway. In addition, PI3K/Akt/mTORC1 signaling pathway may be involved in this process.
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页数：8
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