A fluorescent dye with large Stokes shift and high stability: synthesis and application to live cell imaging

被引：158
|
作者：
Gao, Zheng [1 ,2 ]
Hao, Yongcao [1 ]
Zheng, Meiling [1 ]
Chen, Yi [1 ]
机构：
[1] Chinese Acad Sci, Tech Inst Phys & Chem, Key Lab Photochem Convers & Optoelect Mat, Beijing 100190, Peoples R China
[2] Univ Chinese Acad Sci, Beijing 100190, Peoples R China
来源：
RSC ADVANCES | 2017年 / 7卷 / 13期
基金：
中国国家自然科学基金;
关键词：
CONJUGATED POLYMER NANOPARTICLES; CHEMICAL BIOLOGY; BODIPY DYES; PHOTOSTABILITY; DERIVATIVES; CYANINES; PROBES; WATER; RED; ELECTROLUMINESCENCE;
D O I：
10.1039/c6ra27547h
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A fluorescent dye, 2,5-bis(6-amine-benzoxazol-2-yl) thiophene (BBTA), was synthesized by a two-step reaction from starting material 2,5-bis(benzoxazol-2-yl) thiophene (BBT). BBTA exhibited strong emission and large Stokes shift in solvent, and the largest Stokes shift (Delta lambda = 186 nm or Delta nu = 8572 cm(-1)) was obtained in buffer solution (PBS, pH = 7.2) with 5% (v/v) of polyethylene glycol monomethyl ether (mPEG(550), MW = 550) as additive. Application of BBTA to live cell imaging showed that BBTA was clearly expressed in mitochondria with high contrast. Besides, BBTA showed low cytotoxicity and excellent photo-stability.
引用
收藏
页码：7604 / 7609
页数：6
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