A fluorescent dye with large Stokes shift and high stability: synthesis and application to live cell imaging

被引：166

作者：

Gao, Zheng ^{[1
,2
]}

Hao, Yongcao ^{[1
]}

Zheng, Meiling ^{[1
]}

Chen, Yi ^{[1
]}

机构：

[1] Chinese Acad Sci, Tech Inst Phys & Chem, Key Lab Photochem Convers & Optoelect Mat, Beijing 100190, Peoples R China

[2] Univ Chinese Acad Sci, Beijing 100190, Peoples R China

来源：

RSC ADVANCES | 2017年 / 7卷 / 13期

基金：

中国国家自然科学基金;

关键词：

CONJUGATED POLYMER NANOPARTICLES; CHEMICAL BIOLOGY; BODIPY DYES; PHOTOSTABILITY; DERIVATIVES; CYANINES; PROBES; WATER; RED; ELECTROLUMINESCENCE;

D O I：

10.1039/c6ra27547h

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A fluorescent dye, 2,5-bis(6-amine-benzoxazol-2-yl) thiophene (BBTA), was synthesized by a two-step reaction from starting material 2,5-bis(benzoxazol-2-yl) thiophene (BBT). BBTA exhibited strong emission and large Stokes shift in solvent, and the largest Stokes shift (Delta lambda = 186 nm or Delta nu = 8572 cm(-1)) was obtained in buffer solution (PBS, pH = 7.2) with 5% (v/v) of polyethylene glycol monomethyl ether (mPEG(550), MW = 550) as additive. Application of BBTA to live cell imaging showed that BBTA was clearly expressed in mitochondria with high contrast. Besides, BBTA showed low cytotoxicity and excellent photo-stability.

引用

页码：7604 / 7609

页数：6

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