Bone Marrow Stem Cells and Ear Framework Reconstruction

被引:7
作者
Karimi, Hamid [1 ]
Emami, Seyed-Abolhassan [1 ]
Olad-Gubad, Mohammad-Kazem [2 ]
机构
[1] Iran Univ Med Sci, Sch Med, Hazrat Fatemeh Hosp, Dept Plast & Reconstruct Surg,Fac Med, Tehran, Iran
[2] Iran Univ Med Sci, Sch Med, Hazrat Fatemeh Hosp, Dept Plast & Reconstruct Surg, Tehran, Iran
关键词
Cartilage; ear; microtia; reconstruction; stem cell; CARTILAGE; CHONDROCYTES; REGENERATION;
D O I
10.1097/SCS.0000000000003146
中图分类号
R61 [外科手术学];
学科分类号
摘要
Background:Repair of total human ear loss or congenital lack of ears is one of the challenging issues in plastic and reconstructive surgery.Objective:The aim of the present study was 3D reconstruction of the human ear with cadaveric ear cartilages seeded with human mesenchymal stem cells.Method:We used cadaveric ear cartilages with preserved perichondrium. The samples were divided into 2 groups: group A (cartilage alone) and group B (cartilage seeded with a mixture of fibrin powder and mesenchymal stem cell [1,000,000cells/cm(3)] used and implanted in back of 10 athymic rats). After 12 weeks, the cartilages were removed and shape, size, weight, flexibility, and chondrocyte viability were evaluated. P value <0.05 was considered significant.Results:In group A, size and weight of cartilages clearly reduced (P<0.05) and then shape and flexibility (torsion of cartilages in clockwise and counterclockwise directions) were evaluated, which were found to be significantly reduced (P>0.05). After staining with hematoxylin and eosin and performing microscopic examination, very few live chondrocytes were found in group A. In group B, size and weight of samples were not changed (P<0.05); the shape and flexibility of samples were well maintained (P<0.05) and on performing microscopic examination of cartilage samples, many live chondrocytes were found in cartilage (15-20 chondrocytes in each microscopic field).Conclusion:In samples with human stem cell, all variables (size, shape, weight, and flexibility) were significantly maintained and abundant live chondrocytes were found on performing microscopic examination. This method may be used for reconstruction of full defect of auricles in humans.
引用
收藏
页码:2192 / 2196
页数:5
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