Direct interaction of actin filaments with F-BAR protein pacsin2

被引:46
作者
Kostan, Julius [1 ]
Salzer, Ulrich [1 ,2 ]
Orlova, Albina [3 ]
Toeroe, Imre [1 ]
Hodnik, Vesna [4 ]
Senju, Yosuke [5 ]
Zou, Juan [6 ]
Schreiner, Claudia [1 ]
Steiner, Julia [1 ]
Merilainen, Jari [7 ]
Nikki, Marko [7 ]
Virtanen, Ismo [8 ]
Carugo, Oliviero [1 ,9 ]
Rappsilber, Juri [7 ,10 ]
Lappalainen, Pekka [5 ]
Lehto, Veli-Pekka [7 ]
Anderluh, Gregor [4 ,11 ,12 ]
Egelman, Edward H. [3 ]
Djinovic-Carugo, Kristina [1 ,13 ]
机构
[1] Univ Vienna, Dept Struct & Computat Biol, Max F Perutz Labs, Vienna, Austria
[2] Med Univ Vienna, Dept Med Biochem, Vienna, Austria
[3] Univ Virginia, Med Ctr, Dept Biochem & Mol Genet, Charlottesville, VA USA
[4] Univ Ljubljana, Dept Biol, Biotech Fac, Ljubljana, Slovenia
[5] Univ Helsinki, Inst Biotechnol, Program Cell & Mol Biol, Helsinki, Finland
[6] Univ Edinburgh, Wellcome Trust Ctr Cell Biol, Edinburgh, Midlothian, Scotland
[7] Univ Helsinki, Dept Pathol, Haartman Inst, Helsinki, Finland
[8] Univ Helsinki, Inst Biomed Anat, Helsinki, Finland
[9] Univ Pavia, Dept Chem, I-27100 Pavia, Italy
[10] Technol Univ Berlin, Dept Biotechnol, Berlin, Germany
[11] Natl Inst Chem, Ljubljana, Slovenia
[12] EN FIST Ctr Excellence, Ljubljana, Slovenia
[13] Univ Ljubljana, Dept Biochem, Fac Chem & Chem Technol, Ljubljana, Slovenia
基金
奥地利科学基金会;
关键词
cryo-electron microscopy; F-BAR protein pacsin2; F-actin binding; membrane sculpting; RECEPTOR-MEDIATED ENDOCYTOSIS; MEMBRANE INVAGINATION; DOMAIN SUPERFAMILY; STRUCTURAL BASIS; DYNAMIN; VISUALIZATION; ORGANIZATION; DEFORMATION; ENDOPHILIN; MIGRATION;
D O I
10.15252/embr.201439267
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Two mechanisms have emerged as major regulators of membrane shape: BAR domain-containing proteins, which induce invaginations and protrusions, and nuclear promoting factors, which cause generation of branched actin filaments that exert mechanical forces on membranes. While a large body of information exists on interactions of BAR proteins with membranes and regulatory proteins of the cytoskeleton, little is known about connections between these two processes. Here, we show that the F-BAR domain protein pacsin2 is able to associate with actin filaments using the same concave surface employed to bind to membranes, while some other tested N-BAR and F-BAR proteins (endophilin, CIP4 and FCHO2) do not associate with actin. This finding reveals a new level of complexity in membrane remodeling processes.
引用
收藏
页码:1154 / 1162
页数:9
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