Differential effect of dietary vitamin D supplementation on natural killer cell activity in lean and obese mice

被引:28
作者
Lee, Ga Young [1 ]
Park, Chan Yoon [1 ]
Cha, Kyeong Sun [1 ]
Lee, Seung Eun [1 ]
Pae, Munkyong [2 ]
Han, Sung Nim [1 ,3 ]
机构
[1] Seoul Natl Univ, Dept Food & Nutr, Coll Human Ecol, 1 Gwanak Ro, Seoul 08826, South Korea
[2] Chungbuk Natl Univ, Coll Human Ecol, Dept Food & Nutr, Cheongju, South Korea
[3] Seoul Natl Univ, Res Inst Human Ecol, Seoul, South Korea
基金
新加坡国家研究基金会;
关键词
NK cell; Vitamin D; Obesity; IFN-gamma; CD107a; PROMOTE INSULIN-RESISTANCE; 1,25-DIHYDROXYVITAMIN D-3; ADIPOSE-TISSUE; 25-HYDROXYVITAMIN D; ADAPTIVE IMMUNITY; NK CELLS; T-CELLS; RECEPTOR; EXPRESSION; RESPONSES;
D O I
10.1016/j.jnutbio.2018.01.004
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Vitamin D has an immunoregulatory effect on both innate and adaptive immunity. Contradictory results regarding vitamin D and natural killer (NK) cell functions have been reported with in vitro studies, but little is known about this in vivo. We investigated whether vitamin D levels (50, 1000 or 10,000 IU/kg of diet: DD, DC or DS) affect NK cell functions in mice fed a control or high-fat diet (10% or 45% kcal fat: CD or HFD) for 12 weeks. The splenic NK cell activity was significantly higher in the CD-DS group than the HFD-DS group, and the CD-DS group showed significantly higher NK cell activity compared with the CD-DD and CD-DC groups. However, no difference in NK cell activity was observed among the HFD groups fed different levels of vitamin D. The splenic population of NK cells was significantly higher in the CD-DS group than the HFD-DS group. There was no difference in the intracellular expression of IFN-gamma and the surface expression of NKG2D and CD107a in NK cells by both dietary fat and vitamin D content. The splenic mRNA expression of Ifng and CcI5 was significantly lower in the HFD groups compared with the CD groups, but there was no difference in the mRNA levels of Vdup I and Vdr among the groups. Taken together, these results suggest that dietary vitamin D supplementation can modulate innate immunity by increasing NK activity in control mice but not in obese mice. This effect might be mediated through alternation of the splenic NK cell population. (C) 2018 Elsevier Inc. All rights reserved.
引用
收藏
页码:178 / 184
页数:7
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