Differential gene expression profiling in mucus glands of honey bee (Apis mellifera) drones during sexual maturation

被引：0
作者：
Colonello-Frattini, Ninive Aguiar [2 ]
Hartfelder, Klaus [1 ]
机构：
[1] Univ Sao Paulo, Fac Med Ribeirao Preto, Dept Biol Celular & Mol & Bioagentes Patogen, BR-14049 Ribeirao Preto, Brazil
[2] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Pret, Dept Biol, BR-14049 Ribeirao Preto, Brazil
来源：
APIDOLOGIE | 2009年 / 40卷 / 04期
基金：
巴西圣保罗研究基金会;
关键词：
male accessory gland; Representational Difference Analysis; honeybee; reproduction; deformed wing virus; DROSOPHILA-MELANOGASTER FEMALES; DEFORMED-WING VIRUS; ACID-PHOSPHATASE; 14-3-3; PROTEINS; MATING PLUG; FOS; PHOSPHORYLATION; TRANSCRIPTS; PHYSIOLOGY; COLONIES;
D O I：
10.1051/apido/2009009
中图分类号：
Q96 [昆虫学];
学科分类号：
摘要：
The mating sign that each drone leaves when mating with a queen essentially consists of mucus gland proteins. We employed a Representational Difference Analysis (RDA) methodology to identify genes that are differentially expressed in mucus glands during sexual maturation of drones. The RDA library for mucus glands of newly emerged drones was more complex than that of 8 day-old drones, with matches to 20 predicted genes. Another 26 reads matched to the Apis genome but not to any predicted gene. Since these ESTs were located within ORFs they may represent novel honey bee genes, possibly fast evolving mucus gland proteins. In the RDA library for mucus glands of 8 day-old drones, most reads corresponded to a capsid protein of deformed wing virus, indicating high viral loads in these glands. The expression of two genes encoding venom allergens, acid phosphatase-1 and hyaluronidase, in drone mucus glands argues for their homology with the female venom glands, both associated with the reproductive system.
引用
收藏
页码：481 / 495
页数：15
相关论文
共 58 条
[11]   A MALE ACCESSORY-GLAND PEPTIDE THAT REGULATES REPRODUCTIVE-BEHAVIOR OF FEMALE DROSOPHILA-MELANOGASTER [J].
CHEN, PS ;
STUMMZOLLINGER, E ;
AIGAKI, T ;
BALMER, J ;
BIENZ, M ;
BOHLEN, P .
CELL, 1988, 54 (03) :291-298
[12]   Drosophila Fos mediates ERK and JNK signals via distinct phosphorylation sites [J].
Ciapponi, L ;
Jackson, DB ;
Mlodzik, M ;
Bohmann, D .
GENES & DEVELOPMENT, 2001, 15 (12) :1540-1553
[13]   Proteomic analyses of male contributions to honey bee sperm storage and mating [J].
Collins, A. M. ;
Caperna, T. J. ;
Williams, V. ;
Garrett, W. M. ;
Evans, J. D. .
INSECT MOLECULAR BIOLOGY, 2006, 15 (05) :541-549
[14]   Protein content and pattern during mucus gland maturation and its ecdysteroid control in honey bee drones [J].
Colonello, NA ;
Hartfelder, K .
APIDOLOGIE, 2003, 34 (03) :257-267
[15]  
Costa RAC, 2002, SOCIOBIOLOGY, V40, P403
[16]  
CRUZLANDIM C, 2002, GENET MOL RES, V1, P131
[17]   Top-DER- and Dpp-dependent requirements for the Drosophila fos/kayak gene in follicular epithelium morphogenesis [J].
Dequier, E ;
Souid, S ;
Pál, M ;
Maróy, P ;
Lepesant, JA ;
Yanicostas, C .
MECHANISMS OF DEVELOPMENT, 2001, 106 (1-2) :47-60
[18]   EGene: A configurable pipeline generation system for automated sequence analysis [J].
Durham, AM ;
Kashiwabara, AY ;
Matsunaga, FTG ;
Ahagon, PH ;
Rainone, F ;
Varuzza, L ;
Gruber, A .
BIOINFORMATICS, 2005, 21 (12) :2812-2813
[19]   Community annotation: Procedures, protocols, and supporting tools [J].
Elsik, Christine G. ;
Worley, Kim C. ;
Zhang, Lan ;
Milshina, Natalia V. ;
Jiang, Huaiyang ;
Reese, Justin T. ;
Childs, Kevin L. ;
Venkatraman, Anand ;
Dickens, C. Michael ;
Weinstock, George M. ;
Gibbs, Richard A. .
GENOME RESEARCH, 2006, 16 (11) :1329-1333
[20]   PRECISE ASSESSMENT OF THE NUMBER OF PATRILINES AND OF GENETIC RELATEDNESS IN HONEYBEE COLONIES [J].
ESTOUP, A ;
SOLIGNAC, M ;
CORNUET, JM .
PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1994, 258 (1351) :1-7
123456