Thioflavin T as a fluorescence probe for label-free detection of T4 polynucleotide kinase/phosphatase and its inhibitors

被引：8

作者：

Ma, Changbei ^{[1
,2
,3
]}

Jin, Shunxin ^{[1
,2
]}

Liu, Haisheng ^{[1
,2
]}

Xia, Kun ^{[1
,2
]}

Tang, Jianhua ^{[1
,2
]}

Wang, Kemin ^{[3
]}

Wang, Jun ^{[1
,2
]}

机构：

[1] Cent S Univ, State Key Lab Med Genet, Changsha 410013, Hunan, Peoples R China

[2] Cent S Univ, Sch Life Sci, Changsha 410013, Hunan, Peoples R China

[3] Hunan Univ, State Key Lab Chemo Biosensing & Chemometr, Changsha 410081, Hunan, Peoples R China

来源：

MOLECULAR AND CELLULAR PROBES | 2015年 / 29卷 / 06期

基金：

中国国家自然科学基金;

关键词：

Label-free; Polynucleotide kinase/phosphatase; Thioflavin T; KINASE-ACTIVITY; PHOSPHATASE-ACTIVITY; DNA 3'-PHOSPHATASES; SENSITIVE DETECTION; MOLECULAR BEACONS; STRAND BREAKS; NUCLEIC-ACID; REPAIR; INDUCER; ENZYME;

D O I：

10.1016/j.mcp.2015.11.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed a new methodology for label-free fluorescence turn-on detection of T4 polynucleotide kinase/phosphatase activity (T4 PNKP) using a Thioflavin T probe. This method is very sensitive with a 0.01 unit/mL limit of detection, which is better than those with labeled fluorophores. Furthermore, T4 PNKP inhibition by the inhibitor heparin is shown, demonstrating the potential to screen suitable inhibitor drugs for T4 PNKP. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：500 / 502

页数：3

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