Molecular Mechanism of N,N-Dimethylformamide Degradation in Methylobacterium sp. Strain DM1

被引:41
作者
Lu, Xinyu [1 ,2 ]
Wang, Weiwei [1 ,2 ]
Zhang, Lige [1 ,2 ]
Hu, Haiyang [1 ,2 ]
Xu, Ping [1 ,2 ]
Wei, Tao [3 ]
Tang, Hongzhi [1 ,2 ]
机构
[1] Shanghai Jiao Tong Univ, State Key Lab Microbial Metab, Joint Int Res Lab Metab & Dev Sci, Shanghai, Peoples R China
[2] Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, Shanghai, Peoples R China
[3] Zhengzhou Univ Light Ind, Sch Food & Biol Engn, Zhengzhou, Henan, Peoples R China
基金
美国国家科学基金会; 中国国家自然科学基金;
关键词
Methylobacterium; N; N-dimethylformamidase; N-dimethylformamide; biodegradation; EXTORQUENS AM1; N-DIMETHYLFORMAMIDE; WASTE-WATER; IDENTIFICATION; EXPOSURE; BIODEGRADATION; DEHYDROGENASE; PURIFICATION; METABOLISM; EXPRESSION;
D O I
10.1128/AEM.00275-19
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
N,N-Dimethylformamide (DMF) is one of the most common xenobiotic chemicals, and it can be easily emitted into the environment, where it causes harm to human beings. Herein, an efficient DMF-degrading strain, DM1, was isolated and identified as Methylobacterium sp. This strain can use DMF as the sole source of carbon and nitrogen. Whole-genome sequencing of strain DM1 revealed that it has a 5.66-Mbp chromosome and a 200-kbp megaplasmid. The plasmid pLVM1 specifically harbors the genes essential for the initial steps of DMF degradation, and the chromosome carries the genes facilitating subsequent methylotrophic metabolism. Through analysis of the transcriptome sequencing data, the complete mineralization pathway and redundant gene clusters of DMF degradation were elucidated. The dimethylformamidase (DMFase) gene was heterologously expressed, and DMFase was purified and characterized. Plasmid pLVM1 is catabolically crucial for DMF utilization, as evidenced by the phenotype identification of the plasmid-free strain. This study systematically elucidates the molecular mechanisms of DMF degradation by Methylobacterium. IMPORTANCE DMF is a hazardous pollutant that has been used in the chemical industry, pharmaceutical manufacturing, and agriculture. Biodegradation as a method for removing DMF has received increasing attention. Here, we identified an efficient DMF degrader, Methylobacterium sp. strain DM1, and characterized the complete DMF mineralization pathway and enzymatic properties of DMFase in this strain. This study provides insights into the molecular mechanisms and evolutionary advantage of DMF degradation facilitated by plasmid pLVM1 and redundant genes in strain DM1, suggesting the emergence of new ecotypes of Methylobacterium.
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页数:14
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[31]   Percolative effects of poly(vinylidene fluoride) molecules on C=O group vibrations in N,N-dimethylformamide in molecular assemblies [J].
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[32]   Viscosity and surface tension of binary systems of N,N-dimethylformamide with alkan-1-ols at different temperatures [J].
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Alkhaldi, Khaled H. A. E. ;
AlTuwaim, Mohammad S. ;
Al-Jimaz, Adel S. .
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[33]   Adsorption property and mechanism of PAMAM dendrimer/silica gel hybrids for Fe(III) and Ag(I) from N,N-dimethylformamide [J].
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Qu, Rongjun ;
Chen, Hou ;
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[34]   The essential role of CYP2E1 in metabolism and hepatotoxicity of N,N-dimethylformamide using a novel Cyp2e1 knockout mouse model and a population study [J].
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Liu, Qiang ;
Wang, Lei ;
Zheng, Min ;
Guan, Mingyue ;
Zhang, Man ;
Zhao, Wenjin ;
Wang, Chunmin ;
Lu, Songwen ;
Cheng, Juan ;
Leng, Shuguang .
ARCHIVES OF TOXICOLOGY, 2019, 93 (11) :3169-3181
[35]   Isolation and characterization of a norfloxacin-degrading bacterial strain Aeromonas hydrophila sp. N215-1 [J].
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[36]   Paracoccus denitrificans SD1 mediated augmentation with indigenous mixed cultures for enhanced removal of N,N-dimethylformamide from industrial effluents [J].
Sanjeevkumar, S. ;
Nayak, Anand S. ;
Santoshkumar, M. ;
Siddavattam, D. ;
Karegoudar, T. B. .
BIOCHEMICAL ENGINEERING JOURNAL, 2013, 79 :1-6
[37]   DENSITIES AND EXCESS MOLAR VOLUMES FOR BINARY-MIXTURES OF N,N-DIMETHYLFORMAMIDE PLUS 1,2-DIMETHOXYETHANE [J].
CORRADINI, F ;
FRANCHINI, GC ;
MARCHETTI, A ;
TAGLIAZUCCHI, M ;
TASSI, L ;
TOSI, G .
JOURNAL OF SOLUTION CHEMISTRY, 1994, 23 (07) :777-785
[38]   Formation thermodynamics of binary and ternary lanthanide(III) complexes with 1,10-phenanthroline and chloride in N,N-dimethylformamide [J].
Komiya, M ;
Nishikido, Y ;
Umebayashi, Y ;
Ishiguro, S .
JOURNAL OF SOLUTION CHEMISTRY, 2002, 31 (11) :931-946
[39]   Excess volumes of (N,N-dimethylformamide plus cyclopentanone plus 1-alkanols) at T=303.15K [J].
Venkatesu, P ;
Rao, MVP .
JOURNAL OF CHEMICAL THERMODYNAMICS, 1998, 30 (02) :207-213
[40]   Viscosity and thermodynamics of viscous flow of the systems, 1-propanol plus formamide, plus N-methylformamide, plus N,N-dimethylformamide [J].
Saleh, MA ;
Uddin, MH .
PHYSICS AND CHEMISTRY OF LIQUIDS, 1999, 37 (06) :701-718