Catalytic hairpin assembly indirectly covalent on Fe3O4@C nanoparticles with signal amplification for intracellular detection of miRNA

被引：23

作者：

Fan, Yaofang ^{[1
,2
]}

Liu, Yanming ^{[1
,2
]}

Zhou, Qihui ^{[3
]}

Du, Hao ^{[1
,2
]}

Zhao, Xueyang ^{[1
,2
]}

Ye, Fei ^{[1
,2
]}

Zhao, Huimin ^{[1
,2
]}

机构：

[1] Dalian Univ Technol, Sch Environm Sci & Technol, Key Lab Ind Ecol & Environm Engn, Dalian 116024, Peoples R China

[2] Minist Educ, Beijing, Peoples R China

[3] Qingdao Univ, Inst Translat Med, Dept Stomatol, Affiliated Hosp, Qingdao 266003, Peoples R China

来源：

TALANTA | 2021年 / 223卷

基金：

中国国家自然科学基金;

关键词：

miRNA; Catalytic hairpin assembly; Fe3O4@C nanoparticle; Intracellular visualization detection; Signal amplification; HYBRIDIZATION CHAIN-REACTION; ROLLING-CIRCLE AMPLIFICATION; FREE FLUORESCENCE STRATEGY; MICRORNA DETECTION; SENSITIVE DETECTION; COMPOSITE-MATERIAL; LIVING CELLS; DNA; PROBE; BIOSENSOR;

D O I：

10.1016/j.talanta.2020.121675

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Fluorescence resonance energy transfer, a promising method for in situ imaging of miRNA in living cells, has intrinsic limitation on sensitivity and selectivity. Herein, a fluorescent amplification strategy based on catalyzed hairpin assembly indirectly covalent on Fe3O4@C nanoparticles via short single-stranded DNA was investigated for cellular miRNA detection in living cells, integrating non-enzyme target-active releasing for amplifying the signal output, highly quenching efficiency of Fe3O4@C nanoparticles with low background, ssDNA assisted fluorescent group-fueled chain releasing from Fe3O4@C nanoparticles with enhanced fluorescence response. The designed platform exhibits highly sensitive in a wide linear concentration range of 0.450 pM-190 pM and is highly specific for miRNA-20a detection with the ability of discriminating one mistake base. Additionally, the CHA-Fe3O4@C was successfully applied in imaging visualization of miRNA-20a in the living cell. The strategy provides a promising bioassay approach for clinical research.

引用

页数：9

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