Protein identification and expression analysis using mass spectrometry

被引：29
|
作者：
Kolker, Eugene [1 ]
Higdon, Roger [1 ]
Hogan, Jason M. [1 ]
机构：
[1] BIATECH Inst, Bothell, WA 98011 USA
来源：
TRENDS IN MICROBIOLOGY | 2006年 / 14卷 / 05期
关键词：
D O I：
10.1016/j.tim.2006.03.005
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The identification and quantification of the proteins that a whole organism expresses under certain conditions is a main focus of high-throughput proteomics. Advanced proteomics approaches generate new biologically relevant data and potent hypotheses. A practical report of what proteome studies can and cannot accomplish in common laboratory settings is presented here. The review discusses the most popular tandem mass-spectrometry-based methods and focuses on how to produce reliable results. A step-by-step description of proteome experiments is given, including sample preparation, digestion, labeling, liquid chromatography, data processing, database searching and statistical analysis. The difficulties and bottlenecks of proteome analysis are addressed and the requirements for further improvements are discussed. Several diverse high-throughput proteomics-based studies of microorganisms are described.
引用
收藏
页码：229 / 235
页数：7
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