Fungicidal and cytotoxic activity of a Capsicum chinense defensin expressed by endothelial cells

被引：41

作者：

Anaya-Lopez, Jose L. ^{[1
]}

Lopez-Meza, Joel E. ^{[1
]}

Baizabal-Aguirre, Victor M. ^{[1
]}

Cano-Camacho, Horacio ^{[1
]}

Ochoa-Zarzosa, Alejandra ^{[1
]}

机构：

[1] Univ Michoacana, Fac Med Vet & Zootecnia, Ctr Multidisciplinario Estudios Biotecnol, Adm Chapultepec, Morelia 58262, Michoacan, Mexico

来源：

BIOTECHNOLOGY LETTERS | 2006年 / 28卷 / 14期

关键词：

antimicrobial peptides; Candida albicans; Capsicum chinense; cytotoxic; defensin; endothelial cells;

D O I：

10.1007/s10529-006-9060-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Plant defensins are antimicrobial peptides that exhibit mainly antifungal activity against a broad range of plant fungal pathogens. However, their actions against Candida albicans have not been extensively studied. The mRNA for gamma-thionin, a defensin from Capsicum chinense, has been expressed in bovine endothelial cells. The conditioned medium of these cells showed antifungal activity on germ tube formation (60-70% of inhibition) and on the viability of C. albicans (70-80% of inhibition). Additionally, C. albicans was not able to penetrate transfected cells. Conditioned medium from these cells also inhibited the viability (similar to 80%) of the human tumor cell line, HeLa.

引用

页码：1101 / 1108

页数：8

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