The HIV-1 gp120 causes ultrastructural changes typical of apoptosis in the rat cerebral cortex

被引：58
作者：
Bagetta, G
Corasaniti, MT
Malorni, W
Rainaldi, G
Berliocchi, L
FinazziAgro, A
Nistico, G
机构：
[1] FAC PHARM,CHAIR PHARMACOL,CATANZARO,ITALY
[2] NATL INST HLTH,DEPT ULTRASTRUCT,ROME,ITALY
[3] UNIV ROMA TOR VERGATA,DEPT BIOCHEM,MONDINO TOR VERGATA NEUROBIOL CTR,I-00133 ROME,ITALY
[4] UNIV ROMA TOR VERGATA,DEPT EXPT MED & BIOCHEM SCI,I-00133 ROME,ITALY
来源：
NEUROREPORT | 1996年 / 7卷 / 11期
关键词：
HIV-1; gp120; apoptosis; DNA fragmentation; TUNEL; transmission electron microscopy; rat cerebral cortex;
D O I：
10.1097/00001756-199607290-00005
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
WE used transmission electron microscopy (TEM) and terminal deoxynucleotidyl transferase (TdT)-mediated dUTP-biotin nick end-labelling (TUNEL) techniques to study the neuropathological effects of intracerebroventricular (i.c.v.) injection of recombinant HIV-1 gp 120 in rats. In brain cortical tissue sections from rats treated with a single daily dose of gp120 (100 ng day(-1) for 7 or 14 consecutive days) TEM analysis showed chromatin compaction and marginalization along the inner surface of the nuclear envelope followed by masses of condensed chromatin, ultrastructural signs demonstrating the occurrence of apoptotic cell death. These effects were paralleled by in situ DNA fragmentation, as revealed by application of TUNEL technique to cryostat brain tissue sections from rats treated likewise with the viral coat protein. In no instance was apoptosis seen in the brain cortex of control rats. The present data demonstrate that gp120 given i.c.v. produces apoptosis in the neocortex of rats.
引用
收藏
页码：1722 / 1724
页数：3
相关论文
共 17 条
[1]   The HIV-1 envelope protein GP120 induces neuronal apoptosis in hippocampal slices [J].
AggounZouaoui, D ;
CharriautMarlangue, C ;
Rivera, S ;
Jorquera, I ;
BenAri, Y ;
Represa, A .
NEUROREPORT, 1996, 7 (02) :433-436
[2]   DNA-DEGRADATION IN TERMINALLY DIFFERENTIATING LENS FIBER CELLS FROM CHICK-EMBRYOS [J].
APPLEBY, DW ;
MODAK, SP .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1977, 74 (12) :5579-5583
[3]   HIV-1 GP120 PRODUCES DNA FRAGMENTATION IN THE CEREBRAL-CORTEX OF RAT [J].
BAGETTA, G ;
CORASANITI, MT ;
BERLIOCCHI, L ;
NAVARRA, M ;
FINAZZIAGRO, A ;
NISTICO, G .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1995, 211 (01) :130-136
[4]   INTRACEREBRAL INJECTION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 COAT GLYCOPROTEIN GP120 DOES NOT PRODUCE NEURODEGENERATION IS RATS [J].
BAGETTA, G ;
FINAZZIAGRO, A ;
PALMA, E ;
NISTICO, G .
NEUROSCIENCE LETTERS, 1994, 176 (01) :97-100
[5]  
BAGETTA G, 1996, P NATL ACAD SCI USA, V93, P3256
[6]   HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 COAT PROTEIN NEUROTOXICITY MEDIATED BY NITRIC-OXIDE IN PRIMARY CORTICAL CULTURES [J].
DAWSON, VL ;
DAWSON, TM ;
UHL, GR ;
SNYDER, SH .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (08) :3256-3259
[7]   HIV-1 COAT PROTEIN NEUROTOXICITY PREVENTED BY CALCIUM-CHANNEL ANTAGONISTS [J].
DREYER, EB ;
KAISER, PK ;
OFFERMANN, JT ;
LIPTON, SA .
SCIENCE, 1990, 248 (4953) :364-367
[8]   NEURONAL LOSS IN THE FRONTAL-CORTEX IN HIV-INFECTION [J].
EVERALL, IP ;
LUTHERT, PJ ;
LANTOS, PL .
LANCET, 1991, 337 (8750) :1119-1121
[9]   IDENTIFICATION OF PROGRAMMED CELL-DEATH INSITU VIA SPECIFIC LABELING OF NUCLEAR-DNA FRAGMENTATION [J].
GAVRIELI, Y ;
SHERMAN, Y ;
BENSASSON, SA .
JOURNAL OF CELL BIOLOGY, 1992, 119 (03) :493-501
[10]   HIV ENVELOPE PROTEIN-INDUCED NEURONAL DAMAGE AND RETARDATION OF BEHAVIORAL-DEVELOPMENT IN RAT NEONATES [J].
HILL, JM ;
MERVIS, RF ;
AVIDOR, R ;
MOODY, TW ;
BRENNEMAN, DE .
BRAIN RESEARCH, 1993, 603 (02) :222-233
12