Quantitative measurement of influenza virus replication using consecutive bronchoalveolar lavage in the lower respiratory tract of a ferret model

被引：3

作者：

Lee, Dong-Hun ^{[1
]}

Kim, Jong-In ^{[2
]}

Lee, Jae-Won ^{[2
]}

Chung, Wook-Hun ^{[3
]}

Park, Jae-Keun ^{[1
]}

Lee, Yu-Na ^{[1
]}

Han, Jin Soo ^{[2
]}

Kim, Hwi-Yool ^{[3
]}

Lee, Sang-Won ^{[1
]}

Song, Chang-Seon ^{[1
]}

机构：

[1] Konkuk Univ, Coll Vet Med, Avian Dis Lab, Seoul 143701, South Korea

[2] Konkuk Univ, Coll Vet Med, Dept Lab Anim Med, Seoul 143701, South Korea

[3] Konkuk Univ, Coll Vet Med, Dept Vet Surg, Seoul 143701, South Korea

来源：

JOURNAL OF VETERINARY SCIENCE | 2014年 / 15卷 / 03期

关键词：

bronchoalveolar lavage; ferret; infection; influenza; TRANSMISSION; PATHOGENESIS;

D O I：

10.4142/jvs.2014.15.3.439

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The ferret is an established animal model of influenza virus infection. Although viral replication in the upper respiratory tract is usually measured with consecutively collected nasal washes, daily evaluation of viral replication in the lung is limited because a large numbers of ferrets need to be sacrificed at consecutive time points. To overcome this limitation, we performed a virus quantification assay using bronchoalveolar lavage (BAL) fluid. This non-invasive BAL technique allows consecutive quantification of virus replication in the lungs of living ferrets. Our method can be used for the longitudinal evaluation of virus tropism in the lower respiratory tract.

引用

页码：439 / 442

页数：4

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