Isolation and characterization of twelve polymorphic tetranucleotide microsatellite loci from Yerana yei

被引：1

作者：

Ding, Ling ^{[1
]}

Wang, Hui ^{[1
]}

Pan, Tao ^{[1
]}

Zhou, Wenliang ^{[1
]}

Liu, Zhongquan ^{[2
]}

Zhang, Baowei ^{[1
]}

机构：

[1] Anhui Univ, Sch Life Sci, Hefei 230039, Anhui, Peoples R China

[2] Yancheng Teachers Coll, Sch Life Sci, Yancheng 224000, Jiangsu, Peoples R China

来源：

CONSERVATION GENETICS RESOURCES | 2013年 / 5卷 / 04期

关键词：

Yerana yei; Microsatellite loci; Tetranucleotide; Amphibian; Anuran; Ranidae;

D O I：

10.1007/s12686-013-9928-2

中图分类号：

X176 [生物多样性保护];

学科分类号：

090705 ;

摘要：

Twelve highly polymorphic microsatellite markers were isolated and characterized for the frog Yerana yei. Loci were isolated from a genomic library from Y. yei enriched for (ATAG)(n) repetitive elements. We designed primers that reliably amplified twelve polymorphic loci and tested them on 32 individuals from two populations. The number of alleles per locus ranged from 3-20, the observed and expected heterozygosity ranged within 0.500-1.000 and 0.752-0.964 respectively, and the polymorphic information content (PIC) value ranged from 0.683-0.930. These microsatellite markers could be valuable in future research concerning the conservation genetics and molecular ecology of this species.

引用

页码：899 / 901

页数：3

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