Polymorphic microsatellite primers developed from DNA of the endangered Mekong giant catfish, Pangasianodon gigas (Chevey) and cross-species amplification in three species of Pangasius

被引：9

作者：

Na-Nakorn, Uthairat ^{[1
]}

Sriphairoj, Kednapat

Sukmanomon, Srijanya

Poompuang, Supawadee

Kamonrat, Wongpathom

机构：

[1] Kasetsart Univ, Fac Fisheries, Dept Aquaculture, Bangkok 10900, Thailand

[2] Inland Fisheries Res Inst, Dept Fisheries, Minst Agr & Cooperat, Bangkok 10900, Thailand

来源：

MOLECULAR ECOLOGY NOTES | 2006年 / 6卷 / 04期

关键词：

cross-species amplification; Mekong giant catfish; microsatellite; Pangasianodon gigas; Pangasius spp; primer;

D O I：

10.1111/j.1471-8286.2006.01481.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The critically endangered Pangasianodon gigas is endemic to the Mekong River. Despite its importance, little is known about its genetic diversity and conservation efforts are hampered. Ten polymorphic dinucleotide microsatellite primer pairs were developed from DNA of P. gigas. The analysis of 20 individuals from hatchery stocks using these primers resulted in two to six alleles/locus; H-O = 0.05-0.95; H-E = 0.05-0.81. All but one locus (Pg-3) conformed to Hardy-Weinberg expectation. Eight, six and seven primer pairs were amplified with the DNA from Pangasianodon hypophthalmus, Pangasius larnaudii and Pangasius sanitwongsei, respectively. These markers will be useful for genetic monitoring of wild and hatchery stocks of these pangasiids.

引用

页码：1174 / 1176

页数：3