Epimedium brevicornum Maxim extract relaxes rabbit corpus cavernosum through multitargets on nitric oxide/cyclic guanosine monophosphate signaling pathway

被引：31

作者：

Chiu, J-H

Chen, K-K

Chien, T-M

Chiou, W-F

Chen, C-C

Wang, J-Y

Lui, W-Y

Wu, C-W

机构：

[1] Natl Yang Ming Univ, Sch Med, Inst Tradit Med, Taipei 112, Taiwan

[2] Vet Gen Hosp, Div Gen Surg, Taipei, Taiwan

[3] Cheng Hsiung Rehabil Med Ctr, Taipei, Taiwan

[4] Vet Gen Hosp, Dept Surg, Div Urol, Taipei 11217, Taiwan

[5] Natl Yang Ming Univ, Sch Med, Dept Surg, Taipei 112, Taiwan

[6] Natl Res Inst Chinese Med, Taipei, Taiwan

来源：

INTERNATIONAL JOURNAL OF IMPOTENCE RESEARCH | 2006年 / 18卷 / 04期

关键词：

erectile dysfunction; Epimedium brevicornum; organ bath; cGMP; PDE; 5;

D O I：

10.1038/sj.ijir.3901437

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

Epimedium brevicornum Maxim (EbM) has been reputed to have sexual stimulation effects on males. The study is aimed to test the hypothesis that EbM extracts relaxed the corpus cavernosum (CC) smooth muscle through activation of multitargets on nitric oxide (NO)/cyclic guanosine monophosphate (cGMP) signaling pathway. Water extract of EbM and its subfraction (EP-20) were prepared and standardized by high-performance liquid chromatography. Isolated rabbit CC strips were mounted in organ baths and isometric tension was recorded in the presence or absence of specific inhibitors related to NO/cGMP signaling such as L-N-G-nitro-arginine methyl ester (L-NAME), 1H-[1,2,4]oxadiazolo-[4,3-a] quinoxalin-1-one (ODQ, a guanylyl cyclase inhibitor) or phosphodiesterase 5 (PDE 5) inhibitors. cGMP level was determined in EP-20-treated CC strips. The results showed that EP-20 enriched the content of L-arginine in the process of purification and relaxed the CC smooth muscle precontracted with phenylephrine (PE, 1 mu M) in a concentration-dependent manner. Besides, EP-20 increased the amount of cGMP production in rabbit CC tissues. Coincubation with EP-20 and L-NAME or ODQ significantly decreased EP-20-induced relaxation whereas EP-20 increased sodium nitroprusside-induced relaxation in PE-precontracted CC strips. Besides, EP-20 increased the potency and the duration of the relaxation effects caused by electrical field stimulation. Finally, EP-20 could potentiate PDE 5 inhibitors in relaxation of PE-precontracted CC strips. We concluded that extract of EbM relax the CC smooth muscle through multitargets in NO/cGMP/PDE 5 pathway and might bring into perspective the treatment strategy for those patients with erectile dysfunction.

引用

页码：335 / 342

页数：8

共 28 条

[1] Improvement in erectile dysfunction after neurotrophic factor gene therapy in diabetic rats [J].