Mouse Oocyte Characterization by Electrical Impedance Spectroscopy

被引：0

作者：

Cao, Yuan ^{[1
]}

Floehr, Julia ^{[2
]}

Azarkh, Danyil ^{[1
]}

Schnakenberg, Uwe ^{[1
]}

机构：

[1] Rhein Westfal TH Aachen, Inst Mat Elect Engn 1, Aachen, Germany

[2] Uniklin RWTH Aachen Univ, Helmholtz Inst Biomed Engn, Aachen, Germany

来源：

2022 IEEE SENSORS | 2022年

关键词：

oocyte; hydrodynamic trapping; microfluidic system; electrical impedance spectroscopy; equivalent electric circuit; aspiration; PLASMA-PROTEIN; FETUIN-B;

D O I：

10.1109/SENSORS52175.2022.9967210

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

Artificial fertilization depends on the oocyte quality, especially on the zona pellucida. This gelatinous outer layer becomes soft before and hardens after sperm penetration. Here, we propose a setup that characterizes the stiffness of the zona pellucida of mouse oocytes by electrical impedance spectroscopy. Single oocytes are hydrodynamically trapped at an aperture, which is located between two ring-shaped electrodes. By applying weak negative pressures to the cell trap, the electrical impedance correlates with the stiffness of the zona pellucida.

引用

页数：4

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