A Label-free "Turn-on" Fluorescence Method for Detecting Mercury Ion in Aqueous Solution Based on N-Methyl Mesoporphyrin IX (NMM)/G-quadruplex DNA

被引：16

作者：

Zhang, Xingping ^{[1
]}

Ding, Baomiao ^{[1
]}

Wu, Huawei ^{[1
]}

Wang, Jiujun ^{[1
]}

Yang, Hualin ^{[1
]}

机构：

[1] Yangtze Univ, Coll Life Sci, Jingchu Food Res & Dev Ctr, Jingzhou 434025, Hubei, Peoples R China

来源：

ANALYTICAL SCIENCES | 2017年 / 33卷 / 02期

基金：

中国国家自然科学基金;

关键词：

Mercury ion; G-quadruplex DNA; T-Hg-T mismatches; G-QUADRUPLEX DNA; GOLD NANOPARTICLES; COLORIMETRIC DETECTION; SELECTIVE DETECTION; INORGANIC MERCURY; LIVING CELLS; RICH DNA; HG-II; SENSOR; BIOSENSOR;

D O I：

10.2116/analsci.33.165

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In the present work, a simple design of "turn-on" fluorescence method for mercury ion was developed and explored based on specific T-Hg-T mismatches as a recognizer and N-methyl mesoporphyrin IX (NMM)/G-quadruplex DNA system as a reporter. The titration experiment showed that the mercury-ion concentration and the fluorescence intensity signal change exhibited a consistent linear correlation within the 50 to 500 nM range with a detection limit down to 12.9 nM. In a selectivity experiment, our method showed obvious selectivity against other metal ions, being consistent with other reported detection methods of mercury ion based on the specific reactivity of T-Hg-T mismatches. Our method was then successfully employed to detect mercury ion in pond water with excellent reliability.

引用

页码：165 / 169

页数：5

共 46 条

[1] Anodic stripping voltammetric detection of mercury(II) using Au-PEDOT modified carbon paste electrode [J].