MicroRNA expression profile of bromocriptine-resistant prolactinomas

被引:42
作者
Wu, Zhe Bao [1 ,2 ]
Li, Wei Qiang [3 ]
Lin, Shao Jian [2 ]
Wang, Cheng De [2 ]
Cai, Lin [2 ]
Lu, Jiang Long [2 ]
Chen, Yun Xiang [4 ]
Su, Zhi Peng [2 ]
Shang, Han Bing [1 ]
Yang, Wen Lei [1 ]
Zhao, Wei Guo [1 ]
机构
[1] Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Dept Neurosurg, Shanghai 200025, Peoples R China
[2] Wenzhou Med Univ, Affiliated Hosp 1, Dept Neurosurg, Wenzhou 325000, Peoples R China
[3] Yuyao Peoples Hosp, Dept Neurosurg, Ningbo 315400, Zhejiang, Peoples R China
[4] Jinhua Peoples Hosp, Dept Neurosurg, Jinhua 321000, Peoples R China
基金
中国国家自然科学基金;
关键词
Prolactinoma; Bromocriptine; Resistance; microRNA; microRNA-93; p21; SECRETING PITUITARY-ADENOMAS; DOWN-REGULATION; CANCER-CELLS; OVARIAN-CARCINOMA; MYELOID-LEUKEMIA; CAVERNOUS SINUS; EARLY RELAPSE; TUMOR-GROWTH; FOLLOW-UP; INHIBITOR;
D O I
10.1016/j.mce.2014.07.014
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
MicroRNAs (miRNA) have been implicated in the resistance of tumors to chemotherapy. However, little is known about miRNA expression in bromocriptine-resistant prolactinomas. In this study, 23 prolactinoma samples were classified as bromocriptine-sensitive or -resistant according to the clinical definition of bromocriptine resistance, and their miRNA expression profiles were determined using Solexa sequencing. We found 41 miRNAs that were differentially expressed between the two groups, and 12 of these were validated by stem-loop qRT-PCR. Hsa-mir-93, hsa-mir-17, hsa-mir-22*, hsa-mir-126*, hsa-mir-142-3p, hsa-mir-144*, hsa-mir-486-5p, hsa-mir-451, and hsa-mir-92a were up-regulated and hsa-mir-30a, hsa-mir-382, and hsa-mir-136 were down-regulated in bromocriptine-resistant prolactinomas in comparison with bromocriptine-sensitive prolactinomas. Furthermore, silencing of mir-93 significantly increased the sensitivity of MMQ cells to dopamine agonist treatment. Mir-93 directly affected p21 expression in MMQ cells by targeting the 3'-UTR. Our study is the first to identify a miRNA expression profile associated with bromocriptine-resistant prolactinoma. (C) 2014 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:10 / 18
页数:9
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