Transcription Factor TBX1 Overexpression Induces Downregulation of Proteins Involved in Retinoic Acid Metabolism: A Comparative Proteomic Analysis

被引：21

作者：

Caterino, Marianna ^{[2
,6
]}

Ruoppolo, Margherita ^{[1
,2
]}

Fulcoli, Gabriella ^{[3
,5
]}

Huynth, Tuong ^{[4
]}

Orru, Stefania ^{[6
,7
]}

Baldini, Antonio ^{[1
,3
]}

Salvatore, Francesco ^{[1
,2
]}

机构：

[1] Univ Naples Federico II, Dipartimento Biochim & Biotecnol Med, I-80131 Naples, Italy

[2] CEINGE Biotecnol Avanzate Scarl, Naples, Italy

[3] Telethon Inst Genet & Med TIGEM, CNR, Ist Genet & Biofis, Naples, Italy

[4] Texas A&M Univ, Hlth Sci Ctr, Inst Biosci & Technol, Houston, TX USA

[5] European Sch Mol Med SEMM, Naples, Italy

[6] Fdn SDN IRCCS, Naples, Italy

[7] Univ Napoli Parthenope, Fac Sci Motorie, Naples, Italy

来源：

JOURNAL OF PROTEOME RESEARCH | 2009年 / 8卷 / 03期

关键词：

DIGE; DiGeorge syndrome; proteomics; retinoic acid; T-box containing transcription factor; DIGEORGE-SYNDROME; CARDIOVASCULAR DEFECTS; GEL-ELECTROPHORESIS; PHARYNGEAL ARCH; AORTIC-ARCH; INNER-EAR; IDENTIFICATION; EXPRESSION; GENE; MORPHOGENESIS;

D O I：

10.1021/pr800870d

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

TBX1 haploinsufficiency is considered a major contributor to the del22q11.2/DiGeorge syndrome (DGS) phenotype. We have used proteomic tools to look at all the major proteins involved in the TBX1-mediated pathways in an attempt to better understand the molecular interactions instrumental to its cellular functions. We found more than 90 proteins that could be targeted by TBX1 through different mechanisms. The most interesting observation is that overexpression of TBX1 results in down-regulation of two proteins involved in retinoic acid metabolism.

引用

页码：1515 / 1526

页数：12

共 44 条

[1] Tissue-specific roles of Tbx1 in the development of the outer, middle and inner ear, defective in 22q11DS patients [J].

Arnold, JS ;

Braunstein, EM ;

Ohyama, T ;

Groves, AK ;

Adams, JC ;

Brown, MC ;

Morrow, BE .

HUMAN MOLECULAR GENETICS, 2006, 15 (10) :1629-1639

[2] Dissecting contiguous gene defects:: TBX1 [J].

Baldini, A .

CURRENT OPINION IN GENETICS & DEVELOPMENT, 2005, 15 (03) :279-284

[3] Dynamic and selective interactions of the transcriptional corepressor TIF1β with the heterochromatin protein HP1 isotypes during cell differentiation [J].

Cammas, Florence ;

Janoshazi, Agnes ;

Lerouge, Thierry ;

Losson, Regine .

DIFFERENTIATION, 2007, 75 (07) :627-637

[4] Tbx6, a mouse T-box gene implicated in paraxial mesoderm formation at gastrulation [J].

Chapman, DL ;

Agulnik, I ;

Hancock, S ;

Silver, LM ;

Papaioannou, VE .

DEVELOPMENTAL BIOLOGY, 1996, 180 (02) :534-542

[5] Proteomics in 2002: A year of technical development and wide-ranging applications [J].

Figeys, D .

ANALYTICAL CHEMISTRY, 2003, 75 (12) :2891-2905

[6] Tbx1, a DiGeorge syndrome candidate gene, is regulated by Sonic hedgehog during pharyngeal arch development [J].

Garg, V ;

Yamagishi, C ;

Hu, TH ;

Kathiriya, IS ;

Yamagishi, H ;

Srivastava, D .

DEVELOPMENTAL BIOLOGY, 2001, 235 (01) :62-73

[7] Evaluation of two-dimensional differential gel electrophoresis for proteomic expression analysis of a model breast cancer cell system [J].

Gharbi, S ;

Gaffney, P ;

Yang, A ;

Zvelebil, MJ ;

Cramer, R ;

Waterfield, MD ;

Timms, JF .

MOLECULAR & CELLULAR PROTEOMICS, 2002, 1 (02) :91-98

[8] Protein degradation by the 26S proteasome system in the normal and stressed myocardium [J].

Gomes, Aldrin V. ;

Zong, Chenggong ;

Ping, Peipei .

ANTIOXIDANTS & REDOX SIGNALING, 2006, 8 (9-10) :1677-1691

[9] Dose-dependent interaction of Tbx1 and Crkl and locally aberrant RA signaling in a model of del22q11 syndrome [J].

Guris, DL ;

Duester, G ;

Papaioannou, VE ;

Imamoto, A .

DEVELOPMENTAL CELL, 2006, 10 (01) :81-92

[10] Tbx1 regulates fibroblast growth factors in the anterior heart field through a reinforcing autoregulatory loop involving forkhead transcription factors [J].

Hu, TH ;

Yamagishi, H ;

Maeda, J ;

McAnally, J ;

Yamagishi, C ;

Srivastava, D .

DEVELOPMENT, 2004, 131 (21) :5491-5502

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