Electrochemical Discrimination between G-Quadruplex and Duplex DNA

被引：14

作者：

De Rache, Aurore ^{[1
]}

Doneux, Thomas ^{[1
]}

Buess-Herman, Claudine ^{[1
]}

机构：

[1] Univ Libre Bruxelles, Fac Sci, B-1050 Brussels, Belgium

来源：

ANALYTICAL CHEMISTRY | 2014年 / 86卷 / 16期

基金：

美国国家科学基金会;

关键词：

THROMBIN-BINDING APTAMER; TELOMERIC G-QUADRUPLEX; ION-EXCHANGE BINDING; CRYSTAL-STRUCTURE; K+ SOLUTION; MODIFIED SURFACES; ELECTRON-TRANSFER; GOLD ELECTRODES; METAL-COMPLEXES; QUANTITATION;

D O I：

10.1021/ac500791s

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Analytical tools enabling the discrimination between duplex DNA and G-quadruplex DNA are necessary to unravel the biological function(s) of G-quadruplexes. A methodology relying on the electrochemical response of the electroactive hexaammineruthenium(III) cation at DNA-modified surfaces is presented. A characteristic voltammetric peak is evidenced for all the investigated G-quadruplex sequences, encompassing various types of folding and numbers of quartets. In contrast, no such peak is detected for dsDNA sequences. The occurrence of the voltammetric peak is the consequence of a strong association between the hexaammineruthenium ligand and the surface-immobilized G-quadruplexes. The peak potential points to a significant contribution of nonelectrostatic interactions between the electroactive ligand and G-quadruplexes. The very good efficiency of the discrimination methodology is demonstrated by comparing a G-quadruplex and its corresponding duplex.

引用

页码：8057 / 8065

页数：9

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