Production of probiotic bovine salami using Lactobacillus plantarum 299v as adjunct

被引:41
作者
Blaiotta, Giuseppe [1 ]
Murru, Nicoletta [2 ]
Di Cerbo, Alessandro [3 ]
Romano, Raffaele [1 ]
Aponte, Maria [1 ]
机构
[1] Univ Napoli Federico II, Dipartimento Agr, Via Univ 100, I-80055 Portici, NA, Italy
[2] Univ Napoli Federico II, Dipartimento Med Vet & Prod Anim, Naples, Italy
[3] Univ Modena & Reggio Emilia, Dipartimento Chirurg Gen & Specialita Chirurg, Modena, Italy
关键词
bovine salami; probiotic cultures; PCR-DGGE; RAPD-PCR; sensory analysis; LACTIC-ACID BACTERIA; FERMENTED SAUSAGES; SENSORY CHARACTERISTICS; MICROBIAL DYNAMICS; STARTER CULTURES; IDENTIFICATION; MEAT; STRAINS; MICROORGANISMS; DELIVERY;
D O I
10.1002/jsfa.8717
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
BACKGROUNDFive probiotic lactobacilli were tested, alone or in combination with two commercial starters, to select the most suitable strain for a probiotic bovine salami production. Lactobacillus plantarum 299v was used with both starters, to make salami according to a traditional recipe. Salami obtained by using just the starters and by spontaneous fermentation, served as control. Microbial dynamics, as well as the main physico-chemical parameters, were monitored throughout ripening. The survival of probiotic 299v was confirmed by strains' tracking by means of RAPD-PCR coupled to a culture-independent approach PCR-DGGE-based. RESULTSThe results showed a remarkable viability of the probiotic strain even after 60 days of storage. Experimental salami exhibited the same level of sensory acceptance of control salami, were hygienically safe, and characterised by pH, weight loss and microbiological loads within the ranges conventionally advocated for optimal fermented sausages. CONCLUSIONOutcomes indicate the workable possibility of using second-quality beef cuts for probiotic salami production. (c) 2017 Society of Chemical Industry
引用
收藏
页码:2285 / 2294
页数:10
相关论文
共 31 条
[1]  
[Anonymous], 2003, 10957 UNI
[2]   Lactic acid bacteria occurring during manufacture and ripening of Provolone del Monaco cheese: Detection by different analytical approaches [J].
Aponte, Maria ;
Fusco, Vincenzina ;
Andolfi, Rosamaria ;
Coppola, Salvatore .
INTERNATIONAL DAIRY JOURNAL, 2008, 18 (04) :403-413
[3]   Use of selected autochthonous lactic acid bacteria for Spanish-style table olive fermentation [J].
Aponte, Maria ;
Blaiotta, Giuseppe ;
La Croce, Francesco ;
Mazzaglia, Agata ;
Farina, Vittorio ;
Settanni, Luca ;
Moschetti, Giancarlo .
FOOD MICROBIOLOGY, 2012, 30 (01) :8-16
[4]   Microbial quality and direct PCR identification of lactic acid bacteria and nonpathogenic staphylococci from artisanal low-acid sausages [J].
Aymerich, T ;
Martín, B ;
Garriga, M ;
Hugas, M .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2003, 69 (08) :4583-4594
[5]   Wild game meat: Products, market, legislation and processing controls [J].
Bertolini, R ;
Zgrablic, G ;
Cuffolo, E .
VETERINARY RESEARCH COMMUNICATIONS, 2005, 29 (Suppl 2) :97-100
[6]   Production of fermented chestnut purees by lactic acid bacteria [J].
Blaiotta, G. ;
Di Capua, M. ;
Coppola, R. ;
Aponte, M. .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2012, 158 (03) :195-202
[7]   Commercially standardized process for probiotic "Italico" cheese production [J].
Blaiotta, Giuseppe ;
Murru, Nicoletta ;
Di Cerbo, Alessandro ;
Succi, Mariantonietta ;
Coppola, Raffaele ;
Aponte, Maria .
LWT-FOOD SCIENCE AND TECHNOLOGY, 2017, 79 :601-608
[8]   Optimization of water curing for the preservation of chestnuts (Castanea sativa Mill.) and evaluation of microbial dynamics during process [J].
Blaiotta, Giuseppe ;
Di Capua, Marika ;
Romano, Annalisa ;
Coppola, Raffaele ;
Aponte, Maria .
FOOD MICROBIOLOGY, 2014, 42 :47-55
[9]   Effect of selected dairy starter cultures on microbiological, chemical and sensory characteristics of swine and venison (Dama dama) nitrite-free dry-cured sausages [J].
Cenci-Goga, B. T. ;
Rossitto, P. V. ;
Sechi, P. ;
Parmegiani, S. ;
Cambiotti, V. ;
Cullor, J. S. .
MEAT SCIENCE, 2012, 90 (03) :599-606
[10]   Denaturing gradient gel electrophoresis analysis of the 16S rRNA gene V1 region to monitor dynamic changes in the bacterial population during fermentation of Italian sausages [J].
Cocolin, L ;
Manzano, M ;
Cantoni, C ;
Comi, G .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2001, 67 (11) :5113-5121