Preclinical Assessment of a Fully Automated Multiplex PCR Panel for Detection of Central Nervous System Pathogens

被引：64

作者：

Hanson, K. E. ^{[1
,2
]}

Slechta, E. S. ^{[3
]}

Killpack, J. A. ^{[4
]}

Heyrend, C. ^{[5
]}

Lunt, T. ^{[3
]}

Daly, J. A. ^{[2
,5
]}

Hemmert, A. C. ^{[6
]}

Blaschke, A. J. ^{[4
]}

机构：

[1] Univ Utah, Sch Med, Dept Med, Div Infect Dis, Salt Lake City, UT USA

[2] Univ Utah, Dept Pathol, Clin Microbiol, Salt Lake City, UT USA

[3] ARUP Inst Clin & Expt Pathol, Salt Lake City, UT USA

[4] Univ Utah, Dept Pediat, Div Pediat Infect Dis, Salt Lake City, UT USA

[5] Intermt Primary Childrens Hosp, Salt Lake City, UT USA

[6] BioFire Diagnost LLC, Salt Lake City, UT USA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2016年 / 54卷 / 03期

关键词：

BACTERIAL-MENINGITIS; TIME; ASSAYS;

D O I：

10.1128/JCM.02850-15

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We evaluated a multiplexed PCR panel for the detection of 16 bacterial, viral, and fungal pathogens in cerebrospinal fluid. Panel results were compared to routine testing, and discrepancies were resolved by additional nucleic acid amplification tests or sequencing. Overall, the positive and negative agreements across methods were 92.9% and 91.9%, respectively.

引用

页码：785 / 787

页数：3

共 14 条

[1] Community-acquired bacterial meningitis: Risk stratification for adverse clinical outcome and effect of antibiotic timing [J].

Aronin, SI ;

Peduzzi, P ;

Quagliarello, VJ .

ANNALS OF INTERNAL MEDICINE, 1998, 129 (11) :862-869

[2] Epidemiology, Diagnosis, and Antimicrobial Treatment of Acute Bacterial Meningitis [J].

Brouwer, Matthijs C. ;

Tunkel, Allan R. ;

van de Beek, Diederik .

CLINICAL MICROBIOLOGY REVIEWS, 2010, 23 (03) :467-+

[3]

Hayden RT, 2008, J CLIN MICROBIOL, V46, P157, DOI 10.1128/JCM.01252-07

[4] Evaluation of a New Cryptococcal Antigen Lateral Flow Immunoassay in Serum, Cerebrospinal Fluid and Urine for the Diagnosis of Cryptococcosis: A Meta-Analysis and Systematic Review [J].

Huang, Hua-Rong ;

Fan, Li-Chao ;

Rajbanshi, Bhavana ;

Xu, Jin-Fu .

PLOS ONE, 2015, 10 (05)

[5] Delays in Initiation of Acyclovir Therapy in Herpes Simplex Encephalitis [J].

Hughes, Peter S. ;

Jackson, Alan C. .

CANADIAN JOURNAL OF NEUROLOGICAL SCIENCES, 2012, 39 (05) :644-648

[6] Use of lyophilized standards for the calibration of a newly developed real time PCR assay for human herpes type six (HHV6) variants A and B [J].

Hymas, W ;

Stevenson, J ;

Taggart, EW ;

Hillyard, D .

JOURNAL OF VIROLOGICAL METHODS, 2005, 128 (1-2) :143-150

[7] Description and validation of a novel real-time RT-PCR enterovirus assay [J].

Hymas, Weston C. ;

Aldous, Wade K. ;

Taggart, Edward W. ;

Stevenson, Jeffery B. ;

Hillyard, David R. .

CLINICAL CHEMISTRY, 2008, 54 (02) :406-413

[8] Aseptic meningitis and viral myelitis [J].

Irani, David N. .

NEUROLOGIC CLINICS, 2008, 26 (03) :635-+

[9] Novel DNA probes with low background and high hybridization-triggered fluorescence [J].

Lukhtanov, Eugeny A. ;

Lokhov, Sergey G. ;

Gorn, Vladimir V. ;

Podyminogin, Mikhail A. ;

Mahoney, Walt .

NUCLEIC ACIDS RESEARCH, 2007, 35 (05)

[10] Presentation, time to antibiotics, and mortality of patients with bacterial meningitis at an urban county medical center [J].

Miner, JR ;

Heegaard, W ;

Mapes, A ;

Biros, M .

JOURNAL OF EMERGENCY MEDICINE, 2001, 21 (04) :387-392

← 1 2 →