Direct Patterning of Anti-Human Serum Albumin Antibodies on Aldehyde-Terminated Silicon Nitride Surfaces for HSA Protein Detection

The controlled direct patterning of anti-HSA antibodies on aldehyde-terminated Si3N4 surfaces by a combination of surface functionalization, based on aldehyde-terminated Si3N 4 and μCP was achieved. The experiment involved the oxidization of silicon wafer of 100mm diameter and a Si3N4 layer of 100mm thickness was deposited by low-pressure chemical vapor deposition, and the samples were cleaned with organic solvents. A pattern of amine terminated anti-serum albumin monoclonal antibodies was immobilized on the modified substrate without the use of other reagents. The HSA protein were selectively attached to the patterned monoclonal anti-HSA antibodies and sandwiched with polyclonal anti-HSA as demonstrated by fluorescence images. The procedure allows different kinds of molecules to be used suitable for highly selective, sensitive and fast manufacture of electronic devices for biosensor applications.