Functional expression of human methionine aminopeptidase type 1 in Saccharomyces cerevisiae

被引：9

作者：

Dummitt, B ^{[1
]}

Fei, Y ^{[1
]}

Chang, YH ^{[1
]}

机构：

[1] St Louis Univ, Sch Med, Edward A Doisy Dept Biochem & Mol Biol, St Louis, MO 63104 USA

来源：

PROTEIN AND PEPTIDE LETTERS | 2002年 / 9卷 / 04期

关键词：

protein processing; terminal methionine; zinc finger; methionine aminopeptidase;

D O I：

10.2174/0929866023408607

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We expressed recombinant human methionine aminopeptidase type I (MAP or MetAP) in a map1 null yeast strain to determine the extent of functional complementation between the two proteins. The human MetAP1 protein fully rescued the slow growth phenotype associated with deletion of yeast MetAP1, suggesting that the yeast and human MetAP1 proteins may have similar roles in vivo. Expression of human MetAP1 in yeast has significance in understanding the function of the human protein, studying its in vivo substrate specificity, and developing specific anti-fungal drugs to target yeast MetAP1.

引用

页码：295 / 303

页数：9

共 28 条

[1]

[Anonymous], 1988, Antibodies: A Laboratory Manual

[2] EUKARYOTIC METHIONYL AMINOPEPTIDASES - 2 CLASSES OF COBALT-DEPENDENT ENZYMES [J].

ARFIN, SM ;

KENDALL, RL ;

HALL, L ;

WEAVER, LH ;

STEWART, AE ;

MATTHEWS, BW ;

BRADSHAW, RA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (17) :7714-7718

[3] INVIVO HALF-LIFE OF A PROTEIN IS A FUNCTION OF ITS AMINO-TERMINAL RESIDUE [J].

BACHMAIR, A ;

FINLEY, D ;

VARSHAVSKY, A .

SCIENCE, 1986, 234 (4773) :179-186

[4] PROCESSING OF THE INITIATION METHIONINE FROM PROTEINS - PROPERTIES OF THE ESCHERICHIA-COLI METHIONINE AMINOPEPTIDASE AND ITS GENE STRUCTURE [J].

BENBASSAT, A ;

BAUER, K ;

CHANG, SY ;

MYAMBO, K ;

BOOSMAN, A ;

CHANG, S .

JOURNAL OF BACTERIOLOGY, 1987, 169 (02) :751-757

[5]

BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3

[6] METHIONINE AMINOPEPTIDASE GENE OF ESCHERICHIA-COLI IS ESSENTIAL FOR CELL-GROWTH [J].

CHANG, SYP ;

MCGARY, EC ;

CHANG, S .

JOURNAL OF BACTERIOLOGY, 1989, 171 (07) :4071-4072

[7]

CHANG YH, 1992, J BIOL CHEM, V267, P8007

[8] The specificity in vivo of two distinct methionine aminopeptidases in Saccharomyces cerevisiae [J].

Chen, SP ;

Vetro, JA ;

Chang, YH .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2002, 398 (01) :87-93

[9] Identification of eukaryotic peptide deformylases reveals universality of N-terminal protein processing mechanisms [J].

Giglione, C ;

Serero, A ;

Pierre, M ;

Boisson, B ;

Meinnel, T .

EMBO JOURNAL, 2000, 19 (21) :5916-5929

[10] Methionine aminopeptidase (type 2) is the common target for angiogenesis inhibitors AGM-1470 and ovalicin [J].

Griffith, EC ;

Su, Z ;

Turk, BE ;

Chen, SP ;

Chang, YH ;

Wu, ZC ;

Biemann, K ;

Liu, JO .

CHEMISTRY & BIOLOGY, 1997, 4 (06) :461-471

← 1 2 3 →