Light acclimation and pH perturbations affect photosynthetic performance in Chlorella mass culture

被引:19
作者
Ihnken, Sven [1 ]
Beardall, John [2 ]
Kromkamp, Jacco C. [1 ]
Gomez Serrano, Cintia [3 ]
Torres, Moacir A. [4 ]
Masojidek, Jiri [5 ]
Malpartida, Irene [3 ]
Abdala, Robert [3 ]
Gil Jerez, Celia [3 ]
Malapascua, Jose R. [5 ]
Navarro, Enrique [6 ]
Rico, Rosa M. [3 ]
Peralta, Eduardo [3 ]
Ferreira Ezequil, Joao P. [6 ,7 ]
Lopez Figueroa, Felix [3 ]
机构
[1] Royal Netherlands Inst Sea Res NIOZ, NL-4400 AC Yerseke, Netherlands
[2] Monash Univ, Sch Biol Sci, Clayton, Vic 3800, Australia
[3] Univ Malaga, Fac Ciencias, Dept Ecol, E-29071 Malaga, Spain
[4] Univ Sao Paulo, Inst Quim, Dept Bioquim, Sao Paulo, Brazil
[5] Acad Sci Czech Republ, Inst Microbiol, Trebon 37981, Czech Republic
[6] CSIC, Pyrenean Inst Ecol, Zaragoza 50059, Spain
[7] Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal
来源
AQUATIC BIOLOGY | 2014年 / 22卷
关键词
Chlorella; Mass culture; pH perturbance; Chlorophyll fluorescence; Oxygen production; pH drift; Photosynthesis; Photoprotection; PLASTID TERMINAL OXIDASE; CO2 CONCENTRATING MECHANISMS; MEMBRANE H+-ATPASE; INORGANIC CARBON; DUNALIELLA-TERTIOLECTA; GREEN-ALGA; INTRACELLULAR PH; EXTERNAL PH; GROWTH-RATE; CHLAMYDOMONAS-REINHARDTII;
D O I
10.3354/ab00586
中图分类号
Q17 [水生生物学];
学科分类号
071004 ;
摘要
Chlorella spp. are robust chlorophyte microalgal species frequently used in mass culture. The pH optimum for growth is close to neutrality; at this pH, theoretically little energy is required to maintain homeostasis. In the present study, we grew Chlorella fusca cells in an open, outdoor, thin-layer cascade photobioreactor (TLC), under ambient photon flux at the theoretically preferred pH (7.2), and let the culture pass the exponential growth phase. Using pH drift experiments, we show that an alkalization to pH 9 supported photosynthesis in the TLC. The increased photosynthetic activity under alkaline conditions was a pH-dependent effect, and not a dissolved inorganic carbon (DIC) concentration-or light intensity-dependent effect. Re-acidification (in one step or in increments) lowered gross oxygen production and increased non-photochemical quenching in short-term experiments. Gross oxygen production and electron transport rates in PSII were uncoupled during the pH perturbation experiments. Electron transport rates were only marginally affected by pH, whereas oxygen production rates decreased with acidification. Alternative electron pathways, electron donation at the plastid terminal oxidase and state-transitions are discussed as a potential explanation. Because cell material from the TLC was not operating at maximal capacity, we propose that alkalization can support photosynthesis in challenged TLC systems.
引用
收藏
页码:95 / 110
页数:16
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