Determination of sulphite using an immobilized enzyme with flow injection chemiluminescence detection

被引:11
作者
Yaqoob, M [1 ]
Nabi, A
Waseem, A
Masoom-Yasinzai, M
机构
[1] Univ Baluchistan, Dept Chem, Quetta, Pakistan
[2] Univ Baluchistan, Inst Biochem, Quetta, Pakistan
关键词
chemiluminescence; flow injection analysis; sulphite; immobilized enzyme;
D O I
10.1002/bio.752
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A flow injection method is reported for the determination of sulphite-based on chemiluminescent detection. Hydrogen peroxide is produced from sulphite using on-line covalently bound immobilized sulphite oxidase packed in a mini-column, which was mixed downstream and detected via cobalt(II)-catalysed chemiluminescent oxidation of luminol. The limit of detection (2 x standard deviation of the blank) was 1 x 10(-3) mmol/L with sample throughput 60 h(-1). The calibration data was linear over the range of 0.2-1.0 mmol/L with relative standard deviation (n = 4) in the range 0.9-2.0%. Copyright (C) 2004 John Wiley Sons, Ltd.
引用
收藏
页码:26 / 30
页数:5
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