Leginon: New features and applications

被引:78
作者
Cheng, Anchi [1 ]
Negro, Carl [1 ]
Bruhn, Jessica F. [2 ]
Rice, William J. [3 ,4 ]
Dallakyan, Sargis [1 ]
Eng, Edward T. [1 ]
Waterman, David G. [5 ,6 ]
Potter, Clinton S. [1 ,7 ]
Carragher, Bridget [1 ,7 ]
机构
[1] New York Struct Biol Ctr, Simons Electron Microscopy Ctr, Natl Resource Automated Mol Microscopy, New York, NY 10027 USA
[2] NanoImaging Serv, San Diego, CA USA
[3] NYU, Sch Med, Cryoelectron Microscopy Core, New York, NY USA
[4] NYU, Sch Med, Dept Cell Biol, New York, NY 10016 USA
[5] STFC, Rutherford Appleton Lab, Didcot, Oxon, England
[6] Rutherford Appleton Lab, CCP4, Res Complex Harwell, Didcot, Oxon, England
[7] Columbia Univ, Dept Biochem & Mol Biophys, New York, NY USA
基金
美国国家卫生研究院;
关键词
automation; cryoEM; microED; DATA-COLLECTION; CRYO-EM; ACQUISITION; SYSTEM; TILT;
D O I
10.1002/pro.3967
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Leginon is a system for automated data acquisition from a transmission electron microscope. Here we provide an updated summary of the overall Leginon architecture and an update of the current state of the package. We also highlight a few recent developments to provide some concrete examples and use cases.
引用
收藏
页码:136 / 150
页数:15
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