Biochemical analysis and antitumour effect of Abrus precatorius agglutinin derived peptides in Ehrlich's ascites and B16 melanoma mice tumour model

被引：19

作者：

Behera, Birendra ^{[1
]}

Devi, K. Sanjana P. ^{[1
]}

Mishra, Debasish ^{[2
]}

Maiti, Swatilekha ^{[1
]}

Maiti, Tapas K. ^{[1
]}

机构：

[1] Indian Inst Technol, Dept Biotechnol, Kharagpur 721302, W Bengal, India

[2] Vellore Inst Technol, Vellore 632014, Tamil Nadu, India

来源：

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY | 2014年 / 38卷 / 01期

关键词：

Abrus precatorius; Ehrlich's ascites; B16; melanoma; Agglutinin derived peptides; Anionic anticancer peptides; IN-VIVO; ANTICANCER ACTIVITY; CELLS; GROWTH; MOUSE; PROLIFERATION; MITOCHONDRIA; CARCINOMA; APOPTOSIS; TOXICITY;

D O I：

10.1016/j.etap.2014.06.006

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Anticancer and immunostimulatory properties of tryptic digest peptides of Abrus precatorius agglutinin protein (10kDAGP) have already been reported. Here attempt has been made to further validate anticancer properties of 10kDAGP peptides in Ehrlich's ascites carcinoma (EAC) and B16 melanoma (B16M) bearing mice models and to analyze 10kDAGP by anion exchange chromatography and RP-HPLC for obtaining the bioactive fraction from the total peptide pool. 10kDAGP treatment decreased the tumour pack volume by similar to 82% for EAC and 58.8% for B16M. It also showed increase in ex vivo proliferation of splenocyte and thymocyte isolated from tumour bearing mice and increase in TNF-alpha and Interferon-gamma in splenocyte culture supernatant. From chromatographic analysis it was found that anionic peptide fraction may be responsible for anti-proliferative activities of 10kDAGP. As most anticancer peptides are cationic in nature, further studies regarding bioactivity of anionic peptide fraction may lead to novel anticancer peptides and pathways of action. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：288 / 296

页数：9

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