Fluorescent probe for sensitive discrimination of Hcy and Cys/GSH in living cells via dual-emission

被引：53

作者：

Xu, Shuang ^{[1
]}

Zhou, Junliang ^{[1
]}

Dong, Xiaochun ^{[1
]}

Zhao, Weili ^{[1
,2
]}

Zhu, Quangang ^{[3
]}

机构：

[1] Fudan Univ, Sch Pharm, Shanghai 201203, Peoples R China

[2] Henan Univ, Minist Educ, Key Lab Special Funct Mat, Kaifeng 475004, Peoples R China

[3] Shanghai Dermatol Hosp, Shanghai 200443, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2019年 / 1074卷

基金：

中国国家自然科学基金;

关键词：

Fluorescent probe; Biothiols; Discriminative detection; Dual channels imaging; TURN-ON PROBE; SELECTIVE DETECTION; HYDROGEN-SULFIDE; COLORIMETRIC CHEMOSENSORS; RECENT PROGRESS; HOMOCYSTEINE; CYSTEINE; GLUTATHIONE; CYSTEINE/HOMOCYSTEINE; GSH;

D O I：

10.1016/j.aca.2019.05.008

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Abnormal levels of Cys, Hcy and GSH are associated with various diseases, thus monitoring biothiols is of great significance. In this work, a dual-emission responsive near-infrared fluorescent probe NIR-NBD for detecting Hcy and Cys/GSH was developed based on the conjugation of a dicyanoisophorone based fluorophore (NIR-OH) and 7-nitrobenzofurazan (NBD). To our surprise, the addition of Hcy induced significant fluorescence enhancement at both 549 and 697 nm; while Cys/GSH resulted in major fluorescence emission at 697 nm. The detection limit was determined to be 33.2 nM for Cys, 33.5 nM for Hcy, and 34.4 nM for GSH. Therefore, the probe can be used for discriminative detection of Hcy and Cys/GSH. Moreover, fluorescence imaging of HeLa cells indicated that the probe was cell membrane permeable and could be used for visualizing Hcy and Cys/GSH in living cells. (C) 2019 Published by Elsevier B.V.

引用

页码：123 / 130

页数：8

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